1887

Abstract

The rates of cell division and of protein, DNA and RNA synthesis upon transition of to and from rich medium were examined. The changes in cell morphology (elongation) were also examined by optical and electron microscopy. Upon transfer from poor to rich medium, the rate of synthesis of RNA increased rapidly, followed by an increase in protein synthesis within 3 h and by an increase in DNA synthesis within 7 h; cell division began after a lag of about 10 h. Upon transfer from rich to poor medium, the preshift rates for protein and DNA synthesis changed to postshift rates after 3 h and 7 h, respectively; RNA synthesis stopped immediately, there was a transient fall in total RNA, and synthesis was resumed at a new rate only after 24 h. After the period of adjustment to new medium, the bacteria entered the postshift growth in which cell size, the increase in cell mass (absorbance at 650 nm) and viable counts, and the rates of synthesis of protein, DNA and RNA were constant. Ultrastructural examination of elongated cells during the adjustment period showed that they had septa at different stages of formation, but no evidence of fragmentation was found. It was concluded that cell division in was by binary fission, and that the notion of a life-cycle was not supported by the present findings.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-126-1-77
1981-09-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/126/1/mic-126-1-77.html?itemId=/content/journal/micro/10.1099/00221287-126-1-77&mimeType=html&fmt=ahah

References

  1. Brieger E. M., Fell H. B. 1945; Warm stage observations on the initial development of avian tubercle bacillus, cultivated in embryo extract. Journal of Hygiene 44:158–169
    [Google Scholar]
  2. Brieger E. M., Glauert A. M. 1952; A phase-contrast study of reproduction in mycelial strains of avian tubercle bacilli. Journal of General Microbiology 7:287–294
    [Google Scholar]
  3. Brieger E. M., Glauert A. M., Allen A. M. 1959; Cytoplasmic structure in Mycobacterium leprae.. Experimental Cell Research 18:418–420
    [Google Scholar]
  4. Cooper S. 1969; Cell division and DNA replication following a shift to richer medium. Journal of Molecular Biology 43:1–11
    [Google Scholar]
  5. Cooper S., Helmstetter C. E. 1968; Chromosome replication and the cell division cycle of Escherichia coli B/r. Journal of Molecular Biology 31:519–540
    [Google Scholar]
  6. David H. L. 1978; Regulatory mechanisms in the mycobacteria. Annales de Microbiologie 129A:71–73
    [Google Scholar]
  7. Dische Z. 1953; Qualitative and quantitative colorimetric determination of heptoses. Journal of Biological Chemistry 204:983–997
    [Google Scholar]
  8. Helmstetter C. E., Cooper S. 1968; DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r. Journal of Molecular Biology 31:507–518
    [Google Scholar]
  9. Kjeldgaard N. O., Maaløe O., Schaechter M. 1958; The transition between different physiological states during balanced growth of Salmonella typhimurium.. Journal of General Microbiology 19:607–616
    [Google Scholar]
  10. Maaløe O., Kjeldgaard N. O. 1966 Control of Macromolecular Synthesis. New York:: W. A. Benjamin.;
    [Google Scholar]
  11. McCarthy C. 1970; Spontaneous and induced mutations in Mycobacterium avium.. Infection and Immunity 2:223–228
    [Google Scholar]
  12. McCarthy C. 1971; Electronic counting in growth studies of Mycobacterium avium.. Applied Microbiology 22:546–551
    [Google Scholar]
  13. McCarthy C. 1974; Effect of palmitic acid utilisation on cell division in Mycobacterium avium.. Infection and Immunity 9:363–372
    [Google Scholar]
  14. McCarthy C. 1976; Synthesis and release of sulfolipids by Mycobacterium avium during growth and cell division. Infection and Immunity 14:1241–1252
    [Google Scholar]
  15. McCarthy C. 1978; Ammonium ion requirement for the cell cycle of Mycobacterium avium.. Infection and Immunity 19:304–311
    [Google Scholar]
  16. Olitzki A. L., Davis C. L., Schaefer W. B., Cohn M. L. 1969; Colony variants of avian- battey group mycobacteria intracerebrally injected into mice. Pathologia et microbiologia 34:316–323
    [Google Scholar]
  17. Schaechter M., Maaløe O., Kjeldgaard N. O. 1958; Dependency on medium and temperature of cell size and chemical composition during balanced growth of Salmonella typhimurium.. Journal of General Microbiology 19:592–606
    [Google Scholar]
  18. Schaefer W. B., Davis C. L., Cohn M. L. 1970; Pathogenicity of transparent, opaque and rough variants of Mycobacterium avium in chickens and mice. American Review of Respiratory Disease 102:499–506
    [Google Scholar]
  19. Sud I. J., Schaechter M. 1964; Dependence of the content of cell envelopes on the growth rate of Bacillus megaterium.. Journal of Bacteriology 88:1612–1617
    [Google Scholar]
  20. Whitehouse R. L. S., Benichou J. C., Ryter A. 1977; Procedure for the longitudinal orientation of rod shaped bacteria and the production of a high cell density of procaryotic and eucaryotic cells in thin sections for electron microscopy. Biologie cellulaire 30:155–158
    [Google Scholar]
  21. Wichelhausen R. H., Robinson L. B. 1965; Classification of and drug-susceptibility determinations on cultures of mycobacteria other than M. tuberculosis.. Transactions of the 24th Research Conference on Pulmonary Diseases (U.S. Armed Forces, Chicago): p. 56
    [Google Scholar]
  22. Winder F. G., Rooney S. A. 1970; Effects of nitrogenous components of the medium on the carbohydrate and nucleic acid content of Mycobacterium tuberculosis BCG. Journal of General Microbiology 63:29–39
    [Google Scholar]
  23. Woodley C., David H. L. 1976; Effect of temperature on the rate of transparent to opaque type transition in Mycobacterium avium.. Antimicrobial Agents and Chemotherapy 9:113–119
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-126-1-77
Loading
/content/journal/micro/10.1099/00221287-126-1-77
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error