1887

Abstract

The pleiotropic character of the chain-forming mutant of was found to include leakage of periplasmic enzymes and an abnormal tendency to autolyse. Washed suspensions of cells released murein fragments into the supernatant, and cell extracts from the mutant were richer than those of the wild type in exo---acetylglucosaminidase (187% of the wild-type value) and in soluble endopeptidase (256%) activities, but -acetylmuramoylamidase, ,-carboxypeptidase, ,-carboxypeptidase and transglycosylase were not markedly different. When cells were grown in medium containing 0·58 -sucrose, the chains broke up into rods, the ,-carboxypeptidase activity increased about sixfold and ,-carboxypeptidase about twofold. It is suggested that ,-carboxypeptidase is involved in septum splitting. The results suggest that the triggering of autolysis in depends on the alteration of envelope constituents rather than on an enhanced activity of murein hydrolases.

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1981-09-01
2021-08-05
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References

  1. Beck B. D., Park J. T. 1976; Activity of three murein hydrolases during the cell division cycle of Escherichia coli K-12 as measured in toluene-treated cells. Journal of Bacteriology 126:1250–1260
    [Google Scholar]
  2. Braun V., Wolff H. 1975; Attachment of lipoprotein to murein (peptidoglycan) of Escherichia coli in the presence and absence of penicillin FL 1060. Journal of Bacteriology 123:888–897
    [Google Scholar]
  3. Fan D. P. 1970; Autolysin(s) of Bacillus subtilis as dechaining enzyme. Journal of Bacteriology 103:494–499
    [Google Scholar]
  4. Fein J. E., Rogers H. J. 1976; Autolytic enzyme-deficient mutants of Bacillus subtilis 168. Journal of Bacteriology 127:1427–1442
    [Google Scholar]
  5. Garen A., Levinthal C. 1960; A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase. Biochimica et biophysica acta 38:470–483
    [Google Scholar]
  6. Ghuysen J.-M., Shockman G. D. 1973; Bio-synthesis of peptidoglycan. In Bacterial Membranes and Walls pp. 37–130 Leive L. Edited by New York: M. Dekker.;
    [Google Scholar]
  7. Ghuysen J.-M., Tipper D. J., Strominger J. L. 1966; Enzymes that degrade bacterial cell walls. Methods in Enzymology 8:685–699
    [Google Scholar]
  8. Gorecki M., Bar-Eli A., Burstein Y., Patchornik Y., Chain A. B. 1975; The purification of d-alanine carboxypeptidase from Escherichia coli B on a penicillin-Sepharose column. Biochemical Journal 147:131–137
    [Google Scholar]
  9. Guinand M., Michel G., Tipper D. J. 1974; Appearance of a γ-d-glutamyl(L)mesodiamino- pimelate peptidoglycan hydrolase during sporulation in Bacillus sphaericus. Journal of Bacteriology 120:173–184
    [Google Scholar]
  10. Hartmann R. 1973 Isolierung und Charakterisierung einer penicillinempfindlichen Murein-Endopeptidase aus der Zellwand von E. coli. Dissertation, University of Tübingen, Germany.:
    [Google Scholar]
  11. Hartmann R., Bock-Hennig S. B., Schwarz U. 1974; Murein hydrolases in the envelope of Escherichia coli. European Journal of Biochemistry 41:203–208
    [Google Scholar]
  12. Hinks R. P., Daneo-Moore L., Shockman G. D. 1978a; Cellular autolytic activity in synchronized populations of Streptococcus faecium. Journal of Bacteriology 133:822–829
    [Google Scholar]
  13. Hinks R. P., Daneo-Moore L., Shockman G. D. 1978b; Relationship between cellular autolytic activity, peptidoglycan synthesis, septation, and the cell cycle in synchronized populations of Streptococcus faecium. Journal of Bacteriology 134:1074–1080
    [Google Scholar]
  14. Kitano K., Williamson R., Tomasz A. 1980; Murein hydrolase defect in the β-lactam tolerant mutants of Escherichia coli. FEMS Microbiology Letters 7:133–136
    [Google Scholar]
  15. Leduc M., van Heijenoort J. 1980; Autolysis of Escherichia coli. Journal of Bacteriology 142:52–59
    [Google Scholar]
  16. Leyh-Bouille M., Nakel M., Frère J.-M., Johnson K., Ghuysen J.-M., Nieto M., Perkins H. R. 1972; Penicillin-sensitive dd-carboxypeptidases from Streptomyces strains R39 and K11. Biochemistry 11:1290–1298
    [Google Scholar]
  17. Lugtenberg E. J. J., de Haas-Menger L., Ruyters W. H. M. 1972; Murein synthesis and identification of cell wall precursors of temperature sensitive lysis mutants of Escherichia coli. Journal of Bacteriology 109:326–335
    [Google Scholar]
  18. Matsuhashi M., Maruyama I. N., Takagaki Y., Tamaki S., Nishimura Y., Hirota Y. 1978; Isolation of a mutant of Escherichia coli lacking penicillin-sensitive d-alanine carboxypeptidase IA. Proceedings of the National Academy of Sciences of the United States of America 75:2631–2635
    [Google Scholar]
  19. Mett H., Keck W., Funk A., Schwarz U. 1980; Two different species of murein trans-glycosylase in Escherichia coli. Journal of Bacteriology 144:45–52
    [Google Scholar]
  20. Michel G., Starka J. 1979; Phospholipase A activity with integrated phospholipid vesicles in intact cells of an envelope mutant of Escherichia coli. FEBS Letters 108:261–265
    [Google Scholar]
  21. Michel G., Di Savino D., Starka J. 1977; Phospholipid composition and phenotypic correction of an envC division mutant of Escherichia coli. Journal of Bacteriology 129:145–150
    [Google Scholar]
  22. Mitchell P., Moyle J. 1956; Autolytic ‘protoplast’ release in Bacterium coli. Nature; London: 178993
    [Google Scholar]
  23. Osborn M. J., Gander J. E., Parisi E., Carson J. 1972; Mechanism of assembly of the outer membrane of Salmonella typhimurium. Journal of Biological Chemistry 247:3262–3272
    [Google Scholar]
  24. Pellon G., Michel G. 1979; Identification d’yne nucléotide pyrophosphatase exocellulaire dans le milieu de culture de Streptomyces mediterranei ME/R17. Canadian Journal of Microbiology 25:53–60
    [Google Scholar]
  25. Primosigh J., Pelzer H., Maas D., Weidel W. 1961; Chemical characterization of mucopeptides released from the E. coli B cell wall by enzymic action. Biochimica et biophysica acta 46:68–80
    [Google Scholar]
  26. Rodolakis A., Thomas P., Starka J. 1973; Morphological mutants of Escherichia coli. Isolation and ultrastructure of a chain-forming envC mutant. Journal of General Microbiology 75:409–416
    [Google Scholar]
  27. Rothfield L., Romeo D. 1971; Enzyme reactions in biological membranes. In Structure and Function of Biological Membranes pp. 251–284 Rothfield L. I. Edited by New York: Academic Press.;
    [Google Scholar]
  28. Starka J., Di Savino D., Michel G., Rodolakis A., Thomas P. 1974; Phenotypic expression of an envC division mutant of Escherichia coli. Annales de Microbiologie 125B:227–232
    [Google Scholar]
  29. Starkova Z., Thomas P., Starka J. 1978; Morphological mutants of Escherichia coli. Nature of the permeability barrier in mon and envC cells. Annales de Microbiologie 129A:265–284
    [Google Scholar]
  30. Strominger J. L., Threnn R. H., Scott S. S. 1959; Oxamycin, a competitive antagonist of the incorporation of d-alanine into a uridine nucleotide in Staphylococcus aureus. Journal of the American Chemical Society 81:3803–3804
    [Google Scholar]
  31. Tamura T., Imae Y., Strominger J. L. 1976; Purification to homogeneity and properties of two d-alanine carboxypeptidases I from Escherichia coli. Journal of Biological Chemistry 251:414–423
    [Google Scholar]
  32. van Heijenoort J., Parquet C., Flouret B., van Heijenoort Y. 1975; Envelope-bound N-acetyl-muramyl-l-alanine amidase of Escherichia coli K12.Purification and properties of the enzyme. European Journal of Biochemistry 58:611–619
    [Google Scholar]
  33. Wallenfels K. 1962; β-Galactosidase. Methods in Enzymology 5:212–219
    [Google Scholar]
  34. Wolf-Watz H., Normark S. 1976; Evidence for a role of N-acetylmuramyl-l-alanine amidase in septum separation in Escherichia coli. Journal of Bacteriology 128:580–586
    [Google Scholar]
  35. Yem D. W., Wu H. C. 1976; Isolation of Escherichia coli K-12 mutants with altered levels of β-N-acetylglucosaminidase. Journal of Bacteriology 125:372–373
    [Google Scholar]
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