1887

Abstract

Protoplasts of showed the same ultraviolet killing kinetics as spores. Irradiated protoplasts gave rise to recombinants when they were fused with unirradiated protoplasts of a strain carrying complementary genetic markers. The decline with u. v. fluence in the capacity of irradiated protoplasts to yield recombinants inheriting individual markers was some six time less steep than that of the survival of unfused protoplasts; thus, for example, protoplasts reduced to only 0·01% survival still yielded 10% as many recombinants as unirradiated protoplasts. Each of six widely separated markers of the irradiated parent was inherited independently of the others, with a frequency falling exponentially with u. v. fluence.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-126-1-21
1981-09-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/126/1/mic-126-1-21.html?itemId=/content/journal/micro/10.1099/00221287-126-1-21&mimeType=html&fmt=ahah

References

  1. Baltz R. H. 1978; Genetic recombination in Streptomyces fradiae by protoplast fusion and cell regeneration. Journal of General Microbiology 107:93–102
    [Google Scholar]
  2. Bibb M. J., Freeman R. F., Hopwood D. A. 1977; Physical and genetical characterisation of a second sex factor, SCP2, for Streptomyces coelicolor A3 (2). Molecular and General Genetics 154:155–166
    [Google Scholar]
  3. Delic V., Hopwood D. A., Friend E. J. 1970; Mutagenesis by N-methyl-N′-nitro-N-nitroso- guanidine (NTG) in Streptomyces coelicolor. Mutation Research 9:167–182
    [Google Scholar]
  4. Fodor K., Demiri E., Alföldi L. 1978; Polyethylene glycol-induced fusion of heat-irradiated and living protoplasts of Bacillus megaterium. Journal of Bacteriology 135:68–70
    [Google Scholar]
  5. Godfrey O., Ford L., Huber M. L. B. 1978; Inter-species matings of Streptomyces fradiae with Streptomyces bikiniensis mediated by conventional and protoplast fusion techniques. Canadian Journal of Microbiology 24:994–997
    [Google Scholar]
  6. Harold R. J., Hopwood D. A. 1970a; Ultraviolet-sensitive mutants of Streptomyces coelicolor. I.Phenotypic characterisation. Mutation Research 10:427–438
    [Google Scholar]
  7. Harold R. J., Hopwood D. A. 1970b; Ultraviolet-sensitive mutants of Streptomyces coelicolor. II.Genetics. Mutation Research 10:439–448
    [Google Scholar]
  8. Hopwood D. A. 1967; Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriological Reviews 31:373–403
    [Google Scholar]
  9. Hopwood D. A. 1981a; Possible applications of genetic recombination in the discovery of new antibiotics in actinomycetes. In The Future of Antibiotherapy and Antibiotic Research pp. 407–414 Ninet L., Florent J. Edited by London: Academic Press.;
    [Google Scholar]
  10. Hopwood D. A. 1981b; Genetic studies with bacterial protoplasts. Annual Review of Microbiology 35: (in the Press).
    [Google Scholar]
  11. Hopwood D. A. 1981c; Genetic studies of antibiotics and other secondary metabolites. Symposia of the Society for General Microbiology 31:187–218
    [Google Scholar]
  12. Hopwood D. A. 1981d; Future possibilities for the discovery of new antibiotics by genetic engineering. In Beta-Lactam Antibiotics pp. 585–598 Salton M.R. J., Shockman. G. D. Edited by New York: Academic Press.;
    [Google Scholar]
  13. Hopwood D. A., Chater K. F. 1980; Fresh approaches to antibiotic production. Philosophical Transactions of the Royal Society of London B290:313–328
    [Google Scholar]
  14. Hopwood D. A., Wright H.M. 1978; Bacterial protoplast fusion: recombination in fused protoplasts of Streptomyces coelicolor. Molecular and General Genetics 162:307–317
    [Google Scholar]
  15. Hopwood D. A., Wright H.M. 1979; Factors affecting recombinant frequency in protoplast fusions of Streptomyces coelicolor. Journal of General Microbiology 111:137–143
    [Google Scholar]
  16. Hopwood D. A., Chater K. F., Dowding J. E., Vivian A. 1973; Advances in Streptomyces coelicolor genetics. Bacteriological Reviews 37:371–405
    [Google Scholar]
  17. Hopwood D. A., Wright J. M., Bibb M. J., Cohen S. N. 1977; Genetic recombination through protoplast fusion in Streptomyces. Nature: London: 268171–174
    [Google Scholar]
  18. Ochi K., Katz E. 1978; The possible involvement of a plasmid(s) in actinomycin synthesis by Streptomyces parvulus and Streptomyces antibioticus. Journal of Antibiotics 31:1143–1148
    [Google Scholar]
  19. Ochi K., Hitchcock J.M, Katz E. 1979; High-frequency fusion of Streptomyces parvulus or Streptomyces antibioticus protoplasts induced by polyethylene glycol. Journal of Bacteriology 139:984–992
    [Google Scholar]
  20. Wright W. E. 1978; The isolation of heterokaryons and hybrids by a selective system using irreversible biochemical inhibitors. Experimental Cell Research 112:395–407
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-126-1-21
Loading
/content/journal/micro/10.1099/00221287-126-1-21
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error