1887

Abstract

Synthesis of fructokinase of has been obtained with a preincubated S-30 fraction from and RNA from . The fructokinase formed was identified by its exceptional catalytic activity at pH 10 and by the similar specificity properties of the native enzyme and the enzyme synthesized in vitro. Its presence was confirmed among the radioactive products of protein synthesis by its transition of molecular weight from 20000 to 80000 in the presence of MgATP and by electrophoresis of purified preparations. The presumptive mRNA of fructokinase contained poly(A) sequences of variable length. Fructokinase mRNA was present in when grown with fructose but not with glucose, indicating that the induction of fructokinase is controlled at the level of transcription.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-126-1-203
1981-09-01
2021-05-06
Loading full text...

Full text loading...

/deliver/fulltext/micro/126/1/mic-126-1-203.html?itemId=/content/journal/micro/10.1099/00221287-126-1-203&mimeType=html&fmt=ahah

References

  1. Brawerman G. 1974; The isolation of messenger RNA from mammalian cells. Methods in Enzymology 30:605–612
    [Google Scholar]
  2. Chance B., Maehly A. C. 1955; Assay of catalases and peroxidases. Methods in Enzymology 2:764–775
    [Google Scholar]
  3. Cremer K., Bodemer M., Summers W. 1978; Characterization of the mRNA for herpes simplex virus thymidine kinase by cell-free synthesis of active enzyme. Nucleic Acid Research 5:2333–2344
    [Google Scholar]
  4. Dohan F. C., Rubman R. H., Torriani A. 1971; In vitro synthesis of Escherichia coli alkaline phosphatase monomers. Journal of Molecular Biology 53:469–479
    [Google Scholar]
  5. Habermann V. 1961; The effect of 6-azauracil on microorganisms inhibited by chloramphenicol. Biochimica et biophysica acta 49:204–211
    [Google Scholar]
  6. Halbreich A. 1979; Differential effect of Ca2+ on the translation of yeast mitochondria] and some viral RNA’s in an E. coli cell-free system. Biochemical and Biophysical Research Communications 86:78–87
    [Google Scholar]
  7. Halbreich A., Di Franco A., Grondinsky O., Cosson J., Slonimski P. D. 1975; Translation of mitochondrial RNA from yeast cytoplasmic petite mutants in an E. coli cell-free system. Biochemical and Biophysical Research Communications 64:1286–1292
    [Google Scholar]
  8. Herrlich P., Schweiger M. 1978; Discrimination of messenger RNA. FEBS Letters 87:1–6
    [Google Scholar]
  9. Kalckar H. 1947; Differential spectrophotometry of purine compounds by means of specific enzymes. Journal of Biological Chemistry 167:445–459
    [Google Scholar]
  10. Labarca C., Paigen K. 1977; mRNA-directed synthesis of catalytically active mouse β-glucuronidase in Xenopus oocytes. Proceedings of the National Academy of Sciences of the United States of America 744462–4465
    [Google Scholar]
  11. Merkel C. G., Wood T. G., Lingrel J. B. 1976; Shortening of the poly(A) region of mouse globin messenger RNA. Journal of Biological Chemistry 251:5512–5515
    [Google Scholar]
  12. Ohta N., Sanders M., Newton A. 1978; Characterization of unstable poly(A)-RNA in Caulobacter crescentus. Biochimica et biophysica acta 517:65–75
    [Google Scholar]
  13. Pelham H. R. B. 1978; Translation of encephalo- myocarditis virus RNA in vitro yields an active proteolytic processing enzyme. European Journal of Biochemistry 85:457–462
    [Google Scholar]
  14. Sabater B. 1973; Oxidation in vivo of the -SH groups of Streptomyces violaceoruber fructokinase. FEBS Letters 29:63–65
    [Google Scholar]
  15. Sabater B., Asensio C. 1973; Transport of hexoses in Streptomyces violaceoruber. European Journal of Biochemistry 39:201–205
    [Google Scholar]
  16. Sabater B., DeLa Fuente G. 1975; Kinetic properties and related changes of molecular weight in a fructokinase from Streptomyces violaceoruber. Biochimica et biophysica acta 377:258–270
    [Google Scholar]
  17. Sabater B., Sebastian J., Asensio C. 1972; Identification and properties of an inducible and highly specific fructokinase from Streptomyces violaceoruber. Biochimica et biophvsica acta 284:414–420
    [Google Scholar]
  18. Sano H., Spaeth E., Burton W. G. 1979; Messenger RNA of the large subunit of ribulose- 1,5-bisphosphate carboxylase from Chlamydomonas reinhardi. European Journal of Biochemistry 93:173–180
    [Google Scholar]
  19. Sarkar N., Langley D., Paulus H. 1978; Isolation and characterization of polyadenylate containing RNA from Bacillus brevis. Biochemistry 17:3468–3474
    [Google Scholar]
  20. Sebastián J., Asensio C. 1972; Purification and properties of the mannokinase from Escherichia coli. Archives of Biochemistry and Biophysics 151:227–233
    [Google Scholar]
  21. Uchida T., Egami F. 1968; Ribonuclease T1 from Taka-diastase. Methods in Enzvmology 12:228–239
    [Google Scholar]
  22. Verdier G. 1979; Poly(adenylic acid)-containing RNA of Euglena gracilis during chloroplast development. European Journal of Biochemistry 93:573–580
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-126-1-203
Loading
/content/journal/micro/10.1099/00221287-126-1-203
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error