Synthesis of fructokinase of has been obtained with a preincubated S-30 fraction from and RNA from . The fructokinase formed was identified by its exceptional catalytic activity at pH 10 and by the similar specificity properties of the native enzyme and the enzyme synthesized in vitro. Its presence was confirmed among the radioactive products of protein synthesis by its transition of molecular weight from 20000 to 80000 in the presence of MgATP and by electrophoresis of purified preparations. The presumptive mRNA of fructokinase contained poly(A) sequences of variable length. Fructokinase mRNA was present in when grown with fructose but not with glucose, indicating that the induction of fructokinase is controlled at the level of transcription.


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