A survey was made of the effects of different nucleotides, sugar nucleotides and sugars on mannosyltransferase activity in to assess their possible role in the regulation of mannan synthesis. Mannosyltransferase activity in spheroplast lysate and washed membrane preparations was not markedly affected by sugars, nucleoside monophosphates or by most of the sugar nucleotides tested. GDP and GTP both inhibited enzyme activity. ATP caused a significant stimulation, and analysis of the mannan formed suggested that this was due mainly to increased synthesis of the polysaccharide moiety. GDPglucose was a competitive inhibitor of total mannan synthesis and also inhibited the formation of mannolipids.


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