The phosphoroclastic system was demonstrated in cell-free extracts of Clostridium sporogenes by the production of carbon dioxide, acetyl phosphate, ATP and reduced NAD in the presence of pyruvate. The kinetics of acetyl phosphate production and NAD reduction were investigated. The addition of sodium nitrite to a suspension of C. sporogenes in glucose medium resulted in a rapid decrease in intracellular ATP concentration which was accompanied by an accumulation of pyruvate in the medium. This accumulation of pyruvate was caused by inhibition of the phosphoroclastic system by nitrite. Nitrite inhibits this system by reaction of nitric oxide, formed from nitrite, with the non-haem iron of pyruvate: ferredoxin oxidoreductase.
BrooksJ. B.,
MooreW. E. C.1969; Gas chromatographic analysis of amines and other compounds produced by several species of Clostridium. Canadian Journal of Microbiology 15:1433–1447
DuncanC. L.,
FosterE. M.1968; Effect of sodium nitrite, sodium chloride and sodium nitrate on germination and outgrowth of anaerobic spores. Applied Microbiology 16:406–411
EvansH. J.,
McauliffeC. A.1956; Identification of NO, N2O and N2 as products of the non-enzymic reduction of nitrite by ascorbate or reduced diphosphopyridine nucleotide. In Inorganic Nitrogen Metabolism pp. 189–197McelroyW. P.,
GlassB.
Edited by Baltimore, Maryland, U.S.A.: Johns Hopkins University.;
KoepsellH. J.,
JohnsonM. J.1942; Dissimilation of pyruvic acid by cell-free preparations of Clostridium butylicum. Journal of Biological Chemistry 145:379–386
MattooA. K.,
RaoJ. R.1974; Neurospora fructose-1,6-diphosphate aldolase. Inhibition by sodium pyruvate. Biochemical and Biophysical Research Communications 60:1229–1237
McdonaldC. C.,
PhillipsW. D.,
MowerH. F.1965; An electron spin resonance study of some complexes of iron, nitric oxide, and anionic ligands. Journal of the American Chemical Society 87:3319–3326
MortensonL. E.,
ValentineR. C.,
CarnahanJ. E.1962; An electron transport factor from Clostridium pasteurianum. Biochemical and Biophysical Research Communications 7:448–452
O’LearyV.,
SolbergM.1976; Effect of sodium nitrite inhibition on intracellular thiol groups and on the activity of certain glycolytic enzymes in Clostridium perfringens. Applied and Environmental Microbiology 31:208–212
SimmonsR. J.,
CostilowR. N.1962; Enzymes of glucose and pyruvate catabolism in cells, spores and germinated spores of Clostridium botulinum. Journal of Bacteriology 84:1274–1281
ThauerR. K.,
JungermannK.,
RupprechtE.,
DeckerK.1969; Hydrogen formation from NADH in cell-free extracts of Clostridium kluyveri. Acetyl-CoA requirement and ferredoxin dependence. FEBS Letters 4:108–112
UyedaK.,
RabinowitzJ. C.1971; Pyruvate: ferredoxin oxidoreductase III. Purification and properties of the enzyme. Journal of Biological Chemistry 246:3111–3119
WoolumJ. C.,
TiezziE.,
CommonerB.1968; Electron spin resonance of iron-nitric oxide complexes with amino acids, peptides and proteins. Biochimica et biophysica acta 160:311–320
YarbroughJ. M.,
RakeJ. B.,
EagonR. G.1980; Bacterial inhibitory effects of nitrite: inhibition of active transport, but not of group translocation and of intracellular enzymes. Applied and Environmental Microbiology 39:831–834