1887

Abstract

The utilization of aliphatic amides and their metabolism in the facultatively chemolithotrophic hydrogen bacterium has been investigated. This strain grew on fructose with formamide or acetamide as nitrogen sources, and these substrates induced formamidase. Ammonia repressed formamidase formation. Propionamide, butyramide and valeramide served as carbon and nitrogen sources and induced a different amidase, valeramidase. This enzyme was highly active with valeramide and less active with shorter acylamides but did not hydrolyse formamide. Valeramidase was subject to catabolite repression by fructose and succinate. Mutants altered in the regulation of amidase formation were isolated. A mutant able to grow with formamide and acetamide as carbon source formed formamidase constitutively, and this enzyme could not be repressed by ammonia in this mutant; its growth on formamide was autotrophic. A mutant able to grow with acetamide but not with formamide as carbon source was semi-constitutive for valeramidase; in this mutant the inducer specificity was altered and acetamide was the most potent inducer of valeramidase.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-125-2-367
1981-08-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/125/2/mic-125-2-367.html?itemId=/content/journal/micro/10.1099/00221287-125-2-367&mimeType=html&fmt=ahah

References

  1. Bowien B., Mayer F., Codd G. A., Schlegel H. G. 1976; Purification, some properties and quaternary structure of the d-ribulose 1,5-diphosphate carboxylase of Alcaligenes eutrophus. Archives of Microbiology 110:157–166
    [Google Scholar]
  2. Brammar W. J., Clarke P. H. 1964; Induction and repression of Pseudomonas aeruginosa amidase. Journal of General Microbiology 37:307–319
    [Google Scholar]
  3. Chandra T. S., Shetna Y. I. 1977; Oxalate, formate, formamide, and methanol metabolism in Thiobacillus novellus. Journal of Bacteriology 131:389–398
    [Google Scholar]
  4. Clarke P. H. 1970; The aliphatic amidases of Pseudomonas aeruginosa. Advances in Microbial Physiology 4:179–222
    [Google Scholar]
  5. Clarke P. H. 1972; Biochemical and immunological comparison of aliphatic amidases produced by Pseudomonas species. Journal of General Microbiology 71:241–257
    [Google Scholar]
  6. Clarke P. H., Ornston N. 1975; Metabolic pathways and regulation II. In Genetics and Biochemistry of Pseudomonas pp. 263–340 Clarke P. H., Richmond M. H. Edited by London: John Wiley.;
    [Google Scholar]
  7. Fennewald M., Shapiro J. 1977; Regulatory mutations of the Pseudomonas plasmid alk regulon. Journal of Bacteriology 132:622–627
    [Google Scholar]
  8. Friedrich C. G., Bowien B., Friedrich B. 1979; Formate and oxalate metabolism in Alcaligenes eutrophus. Journal of General Microbiology 115:185–192
    [Google Scholar]
  9. Goldberg I. 1976; Bacterial yields on methanol, methylamine, formaldehyde and formate. Biotechnology and Bioengineering 18:1657–1668
    [Google Scholar]
  10. Hynes M. J., Pateman J. A. 1970; The use of amides as nitrogen sources by Aspergillus nidulans. Journal of General Microbiology 63:317–324
    [Google Scholar]
  11. Lang E., Lang H. 1972; Spezifische Farbreaktion zum direkten Nachweis der Ameisensäure. Zeitschrift für analytische Chemie 260:8–10
    [Google Scholar]
  12. Peel D., Quayle J. R. 1961; Microbial growth on C1-compounds. I. Isolation and characterization of Pseudomonas AM1. Biochemical Journal 81:465–469
    [Google Scholar]
  13. Schlegel H. G., Kaltwasser H., Gottschalk G. 1961; Ein Submersverfahren zur Kultur wasser-stoffoxidierender Bakterien: Wachstumsphysiologische Untersuchungen. Archiv für Mikrobiologie 38:209–222
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-125-2-367
Loading
/content/journal/micro/10.1099/00221287-125-2-367
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error