1887

Abstract

Eight solid stationary phases were examined for their suitability for pyrolysis-gas chromatography (Py-GC) of micro-organisms. With temperature programming these phases offered little advantage over the traditional liquid phase Carbowax 20M, but at an isothermal analysis temperature of 100 °C their use solved many technical problems. Pyrograms were produced containing small numbers of baseline-resolved peaks which eluted within 8 to 25 min. Four to six specimens per hour could be examined with two pyrolysers attached to one chromatograph oven. When a control organism was used to derive normalized results, pyrograms were reproducible with a second column and a second pyrolyser, suggesting that inter-laboratory reproducibility may be possible.

Five different bacterial genera were well discriminated and some differentiation was achieved between different isolates of , but similarity between pyrograms was unrelated to orthodox taxonomic grouping. The best discrimination was achieved with Chromosorb 104, followed by Chromosorb 101 and Tenax-GC. With solid phases and isothermal oven temperatures Py-GC is a promising technique for microbial identification.

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1981-08-01
2024-04-16
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References

  1. Brosseau J. D., Carmichael J. W. 1978; Pyrolysis gas-liquid chromatography applied to a study of variation in Arthroderma tuberculatum. Mycopathologia 63:67–79
    [Google Scholar]
  2. French G. L., Gutteridge C. S., Phillips I. 1980; Pyrolysis gas chromatography of Pseudomonas and Acinetobacter species. Journal of Applied Bacteriology 49:505–516
    [Google Scholar]
  3. Gutteridge C. S., Norris J. R. 1979; The application of pyrolysis techniques to the identification of micro-organisms. Journal of Applied Bacteriology 47:5–43
    [Google Scholar]
  4. Hall R. C., Bennett G. W. 1973; Pyrolysis-gas chromatography of several cockroach species. Journal of Chromatographic Science 11:439–443
    [Google Scholar]
  5. Healy M. J. R. 1973; Handling and interpreting multiple results. Proceedings of the Royal Society B154369–374
    [Google Scholar]
  6. Hollis O. L. 1966; Separation of gaseous mixtures using porous polyaromatic polymer beads. Analytical Chemistry 38:309–316
    [Google Scholar]
  7. Levy R. L., Barney J. E. 1968; High resolution and high sensitivity pyrolysis gas chromatography (PGC) for the identification of paints. In Proceedings of the 2nd National Symposium of Law Enforcement, Science and Technology, Chicago pp. 325–331
    [Google Scholar]
  8. Macfie H. J. H., Gutteridge C. S., Norris J. R. 1978; Use of canonical variates analysis in differentiation of bacteria by pyrolysis gas-liquid chromatography. Journal of General Microbiology 104:67–74
    [Google Scholar]
  9. May R. W., Pearson E. F., Porter J., Scothern M. D. 1973; A reproducible pyrolysis gas-chromatographic system for the analysis of paints and plastics. Analyst 98:364–371
    [Google Scholar]
  10. Needleman W., Stuchbery P. 1977; The identification of micro-organisms by pyrolysis gas- liquid chromatography. In Analytical Pyrolysis pp. 77–88 Jones C. E. R., Cramers C. A. Edited by Amsterdam: Elsevier.;
    [Google Scholar]
  11. Nie N. H., Hull C. H., Jenkin J. G., Steinbrenner K., Bent D. H. 1975 Statistical Package for the Social Sciences New York: McGraw-Hill.;
    [Google Scholar]
  12. Oxborrow G. S., Fields N. D., Puleo J. R. 1976; Preparation of pure microbiological samples for pyrolysis gas-liquid chromatography studies. Applied and Environmental Microbiology 32:306–309
    [Google Scholar]
  13. Oxborrow G. S., Fields N. D., Puleo J. R. 1977a; Pyrolysis gas chromatography of the genus Bacillus: effects of growth media on pyrchromatogram reproducibility. Applied and Environmental Microbiology 33:865–870
    [Google Scholar]
  14. Oxborrow G. S., Fields N. D., Puleo J. R. 1977b; Pyrolysis gas-liquid chromatography studies of the genus Bacillus. Effect of growth time on pyrochromatogram reproducibility. In Analytical Pyrolysis pp. 69–76 Jones C. E. R., Cramers C. A. Edited by Amsterdam: Elsevier.;
    [Google Scholar]
  15. Quinn P. A. 1974; Development of high resolution pyrolysis-gas chromatography for the identification of micro-organisms. Journal of Chromatographic Science 12:796–806
    [Google Scholar]
  16. Reiner E., Ewing W. H. 1968; Chemotaxonomic studies of some Gram-negative bacteria by means of pyrolysis-gas-liquid chromatography. Nature; London: 217191–194
    [Google Scholar]
  17. Sekhon A. S., Carmichael J. W. 1975; Classification of some Gymnoascaceae by pyrolysis-gas-liquid chromatography using added marker compounds. Sabouraudia 13:83–88
    [Google Scholar]
  18. Stack M. V., Donoghue H. D., Tyler J. E. 1978; Discrimination between oral streptococci by pyrolysis gas-liquid chromatography. Applied and Environmental Microbiology 35:45–50
    [Google Scholar]
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