1887

Abstract

The structure of the free zoospores of has been examined by electron microscopy of thin-sectioned and negatively stained preparations. There are up to 15 flagella arranged in two rows. The free end of each flagellum is narrow and its tip does not contain microtubules. The flagella and the cell body are coated with distinct surface layers composed of regular arrays of particles and fibrils, respectively. The cell body contains a variety of inclusions. Near to the flagellar pole there are numerous membrane-bound electron-dense globules about 0·2 to 0·7 μ in diameter, between which are microtubules, particles and small vesicles. In the region of the centrally placed nucleus are arrays of helices of ribosome-like particles. These particles also occur in the form of globular aggregates, each partially enclosed within a membrane. The remainder of the cytoplasm is filled with material resembling glycogen. The zoospores stain positively for glycogen and contain ribonucleasesensitive particulate material which is stained by toluidine blue.

Scanning electron microscopy shows that the zoospores attach to the substrate by the flagellar pole.

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/content/journal/micro/10.1099/00221287-125-2-311
1981-08-01
2021-10-16
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References

  1. Barker D. C., Swales L. S. 1972a; Characteristics of ribosomes during differentiation of trophozoites to cysts in axenic Entamoeba sp. Cell Differentiation 1:297–306
    [Google Scholar]
  2. Barker D. C., Swales L. S. 1972b; Comparison of trophozoite helical polysomes with cyst ribosomogen microcrystals in axenic Entamoeba sp. Cell Differentiation 1:307–315
    [Google Scholar]
  3. Byers B. 1967; Structure and formation of ribosome crystals in hypothermic chick embryo cells. Journal of Molecular Biology 26:155–167
    [Google Scholar]
  4. Byers B. 1971; Chick embryo ribosome crystals: analysis of bonding and functional activity in vitro. Proceedings of the National Academy of Sciences of the United States of America 68440–444
    [Google Scholar]
  5. Crain S. M., Benitez H., Vatter A. E. 1964; Some cytologic effects of salivary nerve-growth factor on tissue cultures of peripheral ganglia. Annals of the New York Academy of Sciences 118:206–231
    [Google Scholar]
  6. Fuller M. S. 1977; The zoospore, hallmark of aquatic fungi. Mycologia 69:1–20
    [Google Scholar]
  7. Ghiara G., Taddei C. 1966; Dati citologici e ultrastrutturali su di un particolare tipo di costituenti basofili del citoplasma di cellule follicolari e di ovociti ovarici di rettili. Bolletino della Società italiana di biologia sperimentale 42:784–788
    [Google Scholar]
  8. Held A. A., Emerson R., Fuller M. S., Gleason F. H. 1969; Blastocladia and Aqualinderella: fermentative water molds with high carbon dioxide optima. Science 165:206–209
    [Google Scholar]
  9. Kusamrarn T., Sobhon P., Bailey G. B. 1975; The mechanism of formation of inhibitor-induced ribosome helices in Entamoeba invadens. Journal of Cell Biology 65:529–539
    [Google Scholar]
  10. Lake J. A., Slayter H. S. 1970; Three-dimensional structure of the chromatoid body of Entamoeba invadens. Nature; London: 2271032–1037
    [Google Scholar]
  11. Lake J. A., Slayter H. S. 1972; Three-dimensional structure of the chromatoid body helix of Entamoeba invadens. Journal of Molecular Biology 66:271–282
    [Google Scholar]
  12. Lake J. A., Sabatini D. D., Nonomura Y. 1974; Ribosome structure as studied by electron microscopy. In Ribosomes pp. 543–557 Nomura M., Tissières A., Lengyel P. Edited by New York: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  13. Lange L., Olson L. W. 1979; The uniflagellate phycomycete zoospore. Dansk botanisk Arkiv 33:7–95
    [Google Scholar]
  14. Morgan R. S., Uzman B. G. 1966; Nature of the packing of ribosomes within chromatoid bodies. Science 152:214–216
    [Google Scholar]
  15. Morgan R. S., Slayter H. S., Weller D. L. 1968; Isolation of ribosomes from cysts of Entamoeba invadens. Journal of Cell Biology 36:45–51
    [Google Scholar]
  16. Orpin C. G. 1975; Studies on the rumen flagellate Neocallimastix frontalis. Journal of General Microbiology 91:249–262
    [Google Scholar]
  17. Orpin C. G. 1977; Invasion of plant tissue in the rumen by the flagellate Neocallimastix frontalis. Journal of General Microbiology 98:423–430
    [Google Scholar]
  18. Orpin C. G., Bountiff L. 1978; Zoospore chemotaxis in the rumen phycomycete Neocallimastix frontalis. Journal of General Microbiology 104:113–122
    [Google Scholar]
  19. Porte A., Zahnd I. P. 1961; Structure fine du follicule ovarien de Lacerti stirpium. Compte rendu des séances de la Societé de biologie 155:1058–1061
    [Google Scholar]
  20. Roberts K. 1974; Crystalline glycoprotein cell walls of algae: their structure, composition and assembly. Philosophical Transactions of the Royal Society B268:129–146
    [Google Scholar]
  21. Rosenbaum R. M., Wittner M. J. 1970; Ultrastructure of bacterized and axenic trophozoites of Entamoeba histolytica with particular reference to helical bodies. Journal of Cell Biology 45:367–382
    [Google Scholar]
  22. Taddei C. 1972; Ribosome arrangement during oogenesis of Lacerta sicula Raf. Experimental Cell Research 70:285–292
    [Google Scholar]
  23. Vavra J., Joyon L. 1966; Étude sur la morphologie, le cycle évolutif et la position systématique de Callimastix cyclopsis, Weissenburg 1912. Protistologica 2:5–16
    [Google Scholar]
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