1887

Abstract

Transfer of the ‘ mutation’ of into 168-type strains does not always lead to simultaneous induction of both chromosomal aberrations (translocation and inversion) of the strains carrying it. A strain was isolated (GSY1835) which possessed new rearrangements. It has some characteristics of the donor parent-a mutation in the locus () which does not revert, a similar effect on tryptophan biosynthesis and loss of linkage between the markers flanking the operon. Unlike the ‘trpE26 strains’, GSY1835 yielded haploid Trp transformants and transductants. Merodiploid Trp clones were also produced, but in a fairly low proportion (about 10%). The genetic structure of GSY1835 was found to differ considerably from that of a ‘ strain’. The strain carries a non-tandem duplication of part of segment I of the chromosome (segment Ib: ), with the two copies in inverted position. This duplication is very stable. Recombination may occur betweeen the two copies. The results fit two configurations for the genetic map of the strain and its derivatives. Map 1 differs from that of strain 168 by the insertion of the second copy of Ib inside the locus. In map 2, introduction of this second copy at the same site is accompanied by an inversion of the lower part of the chromosome (segments C-D-II). The haploid Trp clones have the genetic map of strain 168. Models are proposed to interpret the formation of strain GSY1835 () and the production of the haploid Trp transformants and transductants.

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1981-08-01
2021-07-30
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References

  1. Anagnostopoulos C. 1977; Genetic analysis of Bacillus subtilis strains carrying chromosomal rearrangements. In Modern Trends in Bacterial Transformation and Transfection pp. 211–230 Edited by Portolés A., López R., Espinosa M. Amsterdam: Elsevier/North-Holland Biomedical Press;
    [Google Scholar]
  2. Anagnostopoulos C., Trowsdale J. 1976; Production of merodiploid clones in Bacillus subtilis strains. In Microbiology-1976 pp. 44–57 Edited by schlessinger D. Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  3. Anderson R. P., Roth J. R. 1977; Tandem genetic duplications in phage and bacteria. Annual Review of Microbiology 31:473–505
    [Google Scholar]
  4. Audit C., Anagnostopoulos C. 1972; Production of stable and persistent unstable heterogenotes in a mutant of Bacillus subtilis. In Spores V pp. 117–125 Edited by Halvorson H. O., Hanson R., Campbell L. L. Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  5. Audit C., Anagnostopoulos C. 1973; Genetic studies relating to the production of transformed clones diploid in the tryptophan region of the Bacillus subtilis genome. Journal of Bacteriology 114:18–27
    [Google Scholar]
  6. Audit C., Anagnostopoulos C. 1975; Studies on the size of the diploid region in Bacillus subtilis merozygotes from strains carrying the trpE26 mutation. Molecular and General Genetics 137:337–351
    [Google Scholar]
  7. Burkholder P. R., Giles N. H. 1947; Induced biochemical mutations in Bacillus subtilis. American Journal of Botany 34:345–348
    [Google Scholar]
  8. Chalumeau H., Delobbe A., Gay P. 1978; Biochemical and genetic study of d-glucitol transfer and catabolism in Bacillus subtilis. Journal of Bacteriology 134:920–928
    [Google Scholar]
  9. Gay P. 1979; Thèse Doctorat-ès-sciences, Paris, France.
    [Google Scholar]
  10. Gros F., Gallant J., Weisberg R., Cashel M. 1967; Decryptification of RNA polymerase in whole cells of Escherichia coli. Journal of Molecular Biology 25:555–557
    [Google Scholar]
  11. Henner D. J., Hoch J. A. 1980; The Bacillus subtilis chromosome. Microbiological Reviews 44:57–82
    [Google Scholar]
  12. Jamet C., Anagnostopoulos C. 1969; Etude d’une mutation très faiblement transformable au locus de la thréonine desaminase de Bacillus subtilis. Molecular and General Genetics 105:225–242
    [Google Scholar]
  13. Lepesant-Kejzlarová J., Lepesant J.-A., Walle J., Billault A., Dedonder R. 1975; Revision of the linkage map of Bacillus subtilis 168: indication for circularity of the chromosome. Journal of Bacteriology 121:823–834
    [Google Scholar]
  14. Lipsky R. H., Rosenthal R., Zahler S. A. 1981; Defective specialized transducing phages of Bacillus subtilis phage SPβ that carry the sup-3 or sup-44 gene. Abstracts of the Annual Meeting of the American Society for Microbiology H92:
    [Google Scholar]
  15. Lopez M. E., Ferrari F. A., Siccardi A. G., Mazza G., Polsinelli M. 1976; New purine markers in Bacillus subtilis. Journal of Bacteriology 126:533–535
    [Google Scholar]
  16. Riley M., Anilionis A. 1978; Evolution of the bacterial genome. Annual Review of Microbiology 32:519–560
    [Google Scholar]
  17. Smith O. H., Yanofsky C. 1962; Enzymes involved in the biosynthesis of tryptophan. Methods in Enzymology 5:794–806
    [Google Scholar]
  18. Spizizen J. 1958; Transformation of biochemically deficient strains of Bacillus subtilis by deoxy-ribonucleate. Proceedings of the National Academy of sciences of the United States of America 441072–1078
    [Google Scholar]
  19. Starlinger P. 1977; DNA rearrangements in pro-caryotes. Annual Review of Genetics 11:103–136
    [Google Scholar]
  20. Trowsdale J., Anagnostopoulos C. 1975; Evidence for the translocation of a chromosome segment in Bacillus subtilis strains carrying the trpE26 mutation. Journal of Bacteriology 122:886–898
    [Google Scholar]
  21. Trowsdale J., Anagnostopoulos C. 1976; Differences in the genetic structure of Bacillus subtilis strains carrying the trpE26 mutation and strain 168. Journal of Bacteriology 126:609–618
    [Google Scholar]
  22. Young F. E., Wilson G. A. 1975; Chromosomal map of Bacillus subtilis. In >Spores VI pp. 596–614 Edited by Gerhardt P., Costilow R. N., Sadoff H. L. Washington, D. C.: American Society for Microbiology;
    [Google Scholar]
  23. Zamenhof S. 1957; Preparation and assay of deoxyribonucleic acid from animal tissue. Methods in Enzymology 3:696–708
    [Google Scholar]
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