Transfer, Maintenance and Expression of P Plasmids in Strains of Cowpea Rhizobia Free

Abstract

Seven slow-growing strains of cowpea rhizobia ( sp.) were tested for ability to receive the P plasmids pRD1 and R68.45 from The frequency of transfer was 2 × 10 −2 × 10 for five strains including CB756, but no transfer was detected for the other two. The his-nif segment of pRD1 was lost at high frequency in CB756 transconjugants whilst pRD1 and R68.45 and antibiotic resistance genes were stable through 50–100 generations in non-selective media even though growth rates of CB756 were almost halved in transconjugant clones. Furthermore, plasmids were maintained in three strains tested during passage through root nodules, although there were some differences in symbiotic expression between transconjugants and their wild-type parents. A colour change was effected by CB756 in the presence of 6-cyanopurine and therefore could possibly be used as an indicator of expression in addition to acetylene reduction in culture.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-125-2-233
1981-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/125/2/mic-125-2-233.html?itemId=/content/journal/micro/10.1099/00221287-125-2-233&mimeType=html&fmt=ahah

References

  1. Beringer J. E. 1974; R factor transfer in Rhizobium leguminosarum.. Journal of General Microbiology 84:188–198
    [Google Scholar]
  2. Beringer J. E., Hopwood D. A. 1976; Chromosomal recombination and mapping in Rhizobium leguminosarum.. Nature, London 214:291–293
    [Google Scholar]
  3. Beringer J. E., Hoggan S. A., Johnston A. W. B. 1978; Linkage mapping in Rhizobium leguminosarum by means of R plasmid-mediated recombination. Journal of General Microbiology 104:201–207
    [Google Scholar]
  4. Cannon F. C., Postgate J. R. 1976; Expression of Klebsiella nitrogen fixation (nif) genes in Azotobacter. Nature, London 260:271–272
    [Google Scholar]
  5. Cannon F. C., Dixon R. A., Postgate J. R., Primrose S. B. 1974; Chromosomal integration of Klebsiella nitrogen fixation genes in Escherichia coli.. Journal of General Microbiology 80:227–239
    [Google Scholar]
  6. Cole M. A., Elkan G. H. 1979; Multiple antibiotic resistance in Rhizobium japonicum.. Applied and Environmental Microbiology 37:867–870
    [Google Scholar]
  7. Dale J. W., Smith J. T. 1979; The effect of a plasmid on growth and survival of Escherichia coli.. Antonie van Leeuwenhoek 45:103–111
    [Google Scholar]
  8. Davis B. D., Mingioli E. S. 1950; Mutants of Escherichia coli requiring methionine or vitamin B12. Journal of Bacteriology 60:17–28
    [Google Scholar]
  9. Dixon R., Cannon F., Kondorosi A. 1976; Construction of a P plasmid carrying nitrogen fixation genes from Klebsiella pneumoniae.. Nature, London 260:268–271
    [Google Scholar]
  10. Dixon R., Kennedy C., Kondorosi A., Krishnapillai V., Merrick M. 1977; Complementation analysis of Klebsiella pneumoniae mutants defective in nitrogen fixation. Molecular and General Genetics 157:189–198
    [Google Scholar]
  11. Gibson A. H. 1963; Physical environment and symbiotic nitrogen fixation, 1. The effect of root temperature on recently nodulated Trifolium subterraneum L. plants. Australian Journal of Biological Sciences 16:28–42
    [Google Scholar]
  12. Gibson A. H., Scowcroft W. R., Child J. J., Pagan J. D. 1976; Nitrogenase activity in cultured Rhizobium sp. strain 32H1: nutritional and physical considerations. Archives of Microbiology 108:45–54
    [Google Scholar]
  13. Haas D., Holloway B. W. 1976; R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa.. Molecular and General Genetics 144:243–251
    [Google Scholar]
  14. Johnston A. W. B., Beynon J. L., Buchanan-Wollaston A. V., Setchell S. M., Hirsch P. R., Beringer J. E. 1978a; High frequency transfer of nodulating ability between strains and species of Rhizobium.. Nature, London 276:634–636
    [Google Scholar]
  15. Johnston A. W. B., Setchell S. M., Beringer J. E. 1978b; Interspecific crosses between Rhizobium leguminosarum and R. meliloti: formation of haploid recombinants and of R-primes. Journal of General Microbiology 104:209–218
    [Google Scholar]
  16. Josey D. P., Beynon J. L., Johnston A. W. B., Beringer J. E. 1979; Strain identification in Rhizobium using intrinsic antibiotic resistance. Journal of Applied Bacteriology 46:343–350
    [Google Scholar]
  17. Kondorosi A., Kiss G. B., Forrai T., Vincze E., Banfalvi Z. 1977; Circular linkage map of Rhizobium meliloti chromosome. Nature, London 268:525–527
    [Google Scholar]
  18. Kuykendall L. D. 1979; Transfer of R factors to and between genetically marked sub-lines of Rhizobium japonicum.. Applied and Environmental Microbiology 37:862–866
    [Google Scholar]
  19. Ludwig R. A., Signer E. R. 1977; Glutamine synthetase and control of nitrogen fixation in Rhizobium. Nature, London 267:246–248
    [Google Scholar]
  20. Macneil D., Brill W. J. 1978; 6-Cyanopurine, a color indicator useful for isolating mutants in the nif genes of Klebsiella pneumoniae.. Journal of Bacteriology 136:247–252
    [Google Scholar]
  21. Maier R. J., Brill W. J. 1976; Ineffective and non-nodulating mutant strains of Rhizobium japonicum.. Journal of Bacteriology 127:763–769
    [Google Scholar]
  22. Maier R. J., Campbell N. E. R., Hanus F. J., Simpson F. B., Russell S. A., Evans H. J. 1978; Expression of hydrogenase activity in free-living Rhizobium japonicum.. Proceedings of the National Academy of Sciences of the United States of America 753258–3262
    [Google Scholar]
  23. Nagahari K., Koshikawa T., Sakaguchi K. 1979; Expression of Escherichia coli tryptophan operon in Rhizobium leguminosarum.. Molecular and General Genetics 171:115–119
    [Google Scholar]
  24. Pagan J. D., Child J. J., Scowcroft W. R., Gibson A. H. 1975; Nitrogen fixation by Rhizobium cultured on a defined medium. Nature, London 256:406–407
    [Google Scholar]
  25. Pankhurst C. E., Craig A. S. 1978; Effect of oxygen concentration, temperature and combined nitrogen on the morphology and nitrogenase activity of Rhizobium sp. strain 32H1 in agar culture. Journal of General Microbiology 106:207–219
    [Google Scholar]
  26. Pattison A. C., Skinner F. A. 1974; The effects of antimicrobial substances on Rhizobium spp. and their use in selective media. Journal of Applied Bacteriology 37:239–250
    [Google Scholar]
  27. Postgate J. R., Krishnapillai V. 1977; Expression of Klebsiella nif and his genes in Salmonella typhimurium.. Journal of General Microbiology 98:379–385
    [Google Scholar]
  28. Pühler A., Burkardt H. J. 1978; Fertility inhibition in Rhizobium lupini by the resistance plasmid RP4. Molecular and General Genetics 162:163–171
    [Google Scholar]
  29. Ram J., Kumar S. 1979; Host dependence of RPl-specified resistance to ampicillin: differential expression in Escherichia coli and Rhizobium leguminosarum.. Gene 7:349–353
    [Google Scholar]
  30. Spitzbarth M., Pühler A., Heumann W. 1979; Characterization of plasmids isolated from Rhizobium meliloti. Archives of Microbiology 121:1–7
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-125-2-233
Loading
/content/journal/micro/10.1099/00221287-125-2-233
Loading

Data & Media loading...

Most cited Most Cited RSS feed