1887

Abstract

The proportion of a wild-type population capable of initiating aggregation centres in starving fields of was investigated using mixed populations of a few wild-type amoebae and a large excess of a mutant incapable of initiating aggregation signals but fully capable of responding to them. For this purpose an aggregation-deficient mutant (designated NP160) was isolated that showed high sensitivity to wild-type strains in synergy tests. This mutant formed normal cell-surface cyclic AMP receptors and phosphodiesterases and was fully capable of chemotaxis to cyclic AMP. Although it had an active adenylate cyclase and formed cyclic AMP, it was deficient in the pulsatile regulation of this enzyme and did not initiate or relay pulsatile cyclic AMP signals and did not form cell adhesive contact sites A unless given artificial cyclic AMP pulses. When synergistic mixtures of this mutant and the wild-type were made, the mutant formed fruiting bodies at frequencies indicating that up to 1 in 5 of the wild-type cells could initiate aggregation centres when present at a ratio of between 1 in 500 and 1 in 10000 of the mixed amoebal population.

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1981-08-01
2021-07-25
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References

  1. AlcÂntara F., Monk M. 1974; Signal propa-gation during aggregation in the slime mould Dictyostelium discoideum. . Journal of General Microbiology 85:321–334
    [Google Scholar]
  2. Beug H., Katz F. E., Gerisch G. 1973; Dynamics of antigenic membrane sites relating to cell aggregation in Dictyostelium discoideum. . Journalof Cell Biology 56:647–658
    [Google Scholar]
  3. Bonner J. Y., Barkley D. S., Hall E. M., Kondn T. M., Mason J. W., O’Keefe G., Wolfe P. B. 1969; Acrasin, acrasinase and the sensitivity to acrasin in Dictyostelium discoideum. . Develop-mental Biology 20:72–87
    [Google Scholar]
  4. Bradley S. G., Sussman M., Ennis H. L. 1956; Environmental factors affecting the aggregation of the cellular slime mold Dictyostelium discoideum. . Journalof Protozoology 3:33–38
    [Google Scholar]
  5. Coukell M. B., Chan F. K. 1980; The precocious appearance and activation of an adeny-late cyclase in a raμld develoμlng mutant of Dictyostelium discoideum. . FEBS Letters 110:39–42
    [Google Scholar]
  6. Darmon M., Barrand P., Brachet P., Klein C., Pereiradasilva L. 1977; Phenotyμlc suppression of morphogenetic mutants of Dictyostelium discoideum. . Developmental Biology 58:174–184
    [Google Scholar]
  7. Ennis H. L., Sussman M. 1958a; The initiator cell for slime mold aggregation. Proceedings of the National Academy of sciences of the United States of America 44:401–411
    [Google Scholar]
  8. Ennis H. L., Sussman M. 1958b; Synergistic morphogenesis by mixtures of Dictyostelium discoideum wild-type and aggregateless mutants. Journal of General Microbiology 18:433–449
    [Google Scholar]
  9. Gerisch G. 1961; Zellfunktionen und Zellfunktions- wechsel in der Entwicklung von Dictyostelium discoideum. . Developmental Biology 3:685–724
    [Google Scholar]
  10. Gerisch G., Hess B. 1974; cAMP-controlled oscillations in suspended Dictyostelium cells: their relation to morphogenetic cell interactions. Proceedings of the National Academy of sciences of the United States of America 71:2118–2122
    [Google Scholar]
  11. Gerisch G., Fromm H., Huesgen A., Wick U. 1975; Control of cell-contact sites by cyclic AMP pulses in differentiating Dictyostelium cells. Nature; London: 255547–549
    [Google Scholar]
  12. Gingle A. R., Robertson A. 1976; The development of the relaying competence in Dictyostelium discoideum. . Journalof Cell science 20:21–27
    [Google Scholar]
  13. Green A. A., Newell P. C. 1975; Evidence for the existence of two types of cAMP binding sites in aggregating cells of Dictyostelium discoideum. . Cell 6:129–136
    [Google Scholar]
  14. Gross J. D., Peacey M. J., Trevan D. J. 1976; Signal emission and signal propagation during early aggregation in Dictyostelium discoideum. . Journalof Cell science 22:645–656
    [Google Scholar]
  15. Huesgen A., Gerisch G. 1975; Solubilized contact sites A from cell membranes of Dictyostelium discoideum. . FEBS Letters 56:46–49
    [Google Scholar]
  16. Juliani M. H., Klein C. 1978; A biochemical study of the effects of cAMP pulses on aggregateless mutants of Dictyostelium discoideum. . Developmental Biology 62:162–172
    [Google Scholar]
  17. Katz E. R., Sussman M. 1972; Parasexual recombination in Dictyostelium discoideum: selection of stable diploid heterozygotes and stable haploid segregants. Proceedings of the National Academy of sciences of the United States of America 69:495–498
    [Google Scholar]
  18. Konijn T. M., Raper K. B. 1961; Cell aggregation in Dictyostelium discoideum. . Developmental Biology 3:725–756
    [Google Scholar]
  19. Malchow D., Nagele B., schwarz H., Gerisch G. 1972; Membrane-bound cyclic AMP phosphodiesterase in chemotactically responding cells of Dictyostelium discoideum. . European Journal of Biochemistry 28:136–142
    [Google Scholar]
  20. Mosses D., Williams K. L., Newell P. C. 1975; The use of mitotic crossing-over for genetic analysis in Dictyostelium discoideum: mapμlng of linkage group II. Journal of General Microbiology 90:247–259
    [Google Scholar]
  21. Mullens I. A., Newell P. C. 1978; cAMP binding to cell surface receptors of Dictyostelium. . Differentiation 10:171–176
    [Google Scholar]
  22. Raman R. K. 1976; The autonomous cell in Dictyostelium discoideum. . Journalof Theoretical Biology 59:491–495
    [Google Scholar]
  23. Raman R. K., Hashimoto Y., Cohen M. H., Robertson A. 1976; Differentiation for aggregation in the cellular slime moulds: the emergence of autonomously signalling cells in Dictyostelium discoideum. . Journalof Cell science 21:243–259
    [Google Scholar]
  24. Raper K. B. 1935; Dictyostelium discoideum, a new species of slime mold from decaying forest leaves. Journal of Agricultural Research 50:135–147
    [Google Scholar]
  25. Ratner D. I., Newell P. C. 1978; Linkage analysis in Dictyostelium discoideum using multiply marked tester strains: establishment of linkage group VII and the reassessment of earlier linkage data. Journal of General Microbiology 109:225–236
    [Google Scholar]
  26. Ross F. M., Newell P. C. 1979; Genetics of aggregation pattern mutations in the cellular slime mould Dictyostelium discoideum. . Journalof General Microbiology 115:289–300
    [Google Scholar]
  27. Ross F. M., Newell P. C. 1981; Streamers: chemotactic mutants of Dictyostelium with altered cyclic GMP metabolism. Journal of General Microbiology 127: in the Press
    [Google Scholar]
  28. Sussman M. 1952; An analysis of the aggregation phase in the development of the slime molds Dictyosteliaceae. II, Aggregative centre formation by mixtures of Dictyostelium discoideum wild type and aggregateless variants. Biological Bulletin 103:446–457
    [Google Scholar]
  29. Sussman M., Noel E. 1952; An analysis of the aggregation phase in the development of the slime molds Dictyosteliaceae. I, Population distribution of the capacity to initiate aggregation. Biological Bulletin 103:259–268
    [Google Scholar]
  30. Williams K. L., Newell P. C. 1976; A genetic study of aggregation in the cellular slime mould Dictyostelium discoideum using complementation analysis. Genetics 82:287–307
    [Google Scholar]
  31. Williams K. L., Kessin R. H., Newell P. C. 1974; Parasexual genetics in Dictyostelium discoideum: mitotic analysis of acriflavin resistance and growth in axenic medium. Journal of General Microbiology 84:59–69
    [Google Scholar]
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