@article{mbs:/content/journal/micro/10.1099/00221287-125-1-73, author = "Best, D. J. and Higgins, I. J.", title = "Methane-oxidizing Activity and Membrane Morphology in a Methanolgrown Obligate Methanotroph, Methylosinus trichosporium OB3b", journal= "Microbiology", year = "1981", volume = "125", number = "1", pages = "73-84", doi = "https://doi.org/10.1099/00221287-125-1-73", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-125-1-73", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "The obligate methylotroph Methylosinus trichosporium OB3b was capable of growth on methanol as sole carbon source at concentrations as high as 4% (v/v), and viability was maintained over many successive transfers. Methane mono-oxygenase, detected by epoxidizing and hydroxylating activity, was retained. The gross morphology of the organism on this substrate was dependent on culture conditions. It varied from organisms containing extensive peripheral membrane systems to those with extensive inclusions, the latter representing a poorly developed membrane system which predominated under most growth conditions.", }