@article{mbs:/content/journal/micro/10.1099/00221287-125-1-47, author = "Edwards, C. and Mccann, R. J.", title = "Differential Effects of Inhibitors on Respiratory Activity of Synchronous Cultures of Bacillus subtilis Prepared by Continuous-flow Centrifugation", journal= "Microbiology", year = "1981", volume = "125", number = "1", pages = "47-53", doi = "https://doi.org/10.1099/00221287-125-1-47", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-125-1-47", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Size selection by continuous-flow centrifugation was used to prepare synchronous cultures of Bacillus subtilis. A selection of 5–10% was required to yield cultures which gave synchrony indices of about 0·45. Respiration rates [nmol O2 min−1 (ml culture)−1] of cells growing in the presence of glucose rose discontinuously as two steps in each cell cycle with the mid-points of the rises centred at 0·37 and 0·87 of a cycle. An asynchronous control culture exhibited no such periodicities. Two inhibitors of energy conservationN,N′-dicyclohexylcarbodi-imide and 4-chloro-7-nitrobenzofurazaninhibited respiration maximally at 0·0 and 0·6 of a cycle whilst an inhibitor of respiration2-heptyl-4-hydroxyquinoline-N-oxideexerted maximal effect only at 0·4 of a cycle. These results are compared with other temporal events which have been reported to occur during the cell cycle of B. subtilis.", }