1887

Abstract

Size selection by continuous-flow centrifugation was used to prepare synchronous cultures of . A selection of 5–10% was required to yield cultures which gave synchrony indices of about 0·45. Respiration rates [nmol O min (ml culture)] of cells growing in the presence of glucose rose discontinuously as two steps in each cell cycle with the mid-points of the rises centred at 0·37 and 0·87 of a cycle. An asynchronous control culture exhibited no such periodicities. Two inhibitors of energy conservation-dicyclohexylcarbodi-imide and 4-chloro-7-nitrobenzofurazaninhibited respiration maximally at 0·0 and 0·6 of a cycle whilst an inhibitor of respiration2-heptyl-4-hydroxyquinoline--oxideexerted maximal effect only at 0·4 of a cycle. These results are compared with other temporal events which have been reported to occur during the cell cycle of .

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/content/journal/micro/10.1099/00221287-125-1-47
1981-07-01
2021-05-18
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