1887

Abstract

Glucose, galactose and fructose uptake was studied during the germination of spores. Dormant spores had only the glucose uptake system, whatever the carbon source of the sporulation medium. Even when sporulation took place in the presence of galactose or fructose as the sole carbon and energy source neither galactose nor fructose uptake was detected. During germination the uptake systems for these two sugars were inducible by the appropriate sugar; the induction time was less when the germination process was more advanced. Synthesis of all uptake systems was immediately blocked by the addition of inhibitors of protein or RNA synthesis. Vegetative mycelium in the exponential phase of growth showed a similar pattern of sugar uptake. Glucose caused repression of the galactose and fructose uptake systems. The three sugar uptake systems were strongly inhibited by potassium cyanide, 2,4-dinitrophenol and -chloromercuribenzoate, and showed optimum temperatures for uptake at 40°C (glucose) and 45 °C (galactose and fructose).

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/content/journal/micro/10.1099/00221287-125-1-25
1981-07-01
2021-07-23
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References

  1. Attwell R. W., Cross T. 1973; Germination of actinomycete spores. In The Actinomycetales: Characteristics and Practical Importance pp. 197–207 Sykes G., Skinner F. A. Edited by London & New York: Academic Press.;
    [Google Scholar]
  2. Gross W., Burkhardt K. L. 1973; Multiple transport systems for basic amino acid transport in Streptomyces hydrogenans. Biochimica et biophysica acta 298:437–445
    [Google Scholar]
  3. Hardisson C., Manzanal M. B., Salas J. A., Suárez J. E. 1978; Fine structure, physiology and biochemistry of arthrospore germination in Streptomyces antibioticus. Journal of General Microbiology 105:203–214
    [Google Scholar]
  4. Hardisson C., Salas J. A., Guuarro J. A., Suárez J. E. 1980; Macromolecular synthesis during germination of Streptomyces spores in a chemically defined medium. FEMS Microbiology Letters 7:233–235
    [Google Scholar]
  5. Hirsch C. F., Ensign J. C. 1975; Germination of Streptomyces viridochromogenes spores. In Spores VI pp. 28–35 Gerhardt P., Costilow R. N., Sadoff H. L. Edited by Washington, D.C.: American Society for Microbiology.;
    [Google Scholar]
  6. Hirsch C. F., Ensign J. C. 1976a; Nutritionally defined conditions for germination of Streptomyces viridochromogenes spores. Journal of Bacteriology 126:13–23
    [Google Scholar]
  7. Hirsch C. F., Ensign J. C. 1976b; Heat activation of Streptomyces viridochromogenes spores. Journal of Bacteriology 126:24–30
    [Google Scholar]
  8. Kalakoutskii L. V., Pouzharitskaja L. M. 1973; The Streptomyces spore: its distinct features and germinal behaviour. In The Actinomycetales: Characteristics and Practical Importance pp. 155–178 Sykes G., Skinner F. A. Edited by London & New York: Academic Press.;
    [Google Scholar]
  9. Ring K., Ehle H., Foit B. 1976; Effect of alkali ions on the active transport of neutral amino acids into Streptomyces hydrogenans. Biochimica et biophysica acta 433:615–629
    [Google Scholar]
  10. Ring K., Gross W., Ehle H., Foit B. 1977; Characterization of l-aspartate uptake by Streptomyces hydrogenans. Journal of General Microbiology 103:307–317
    [Google Scholar]
  11. Sabater B., Asensio C. 1973; Transport of hexoses in Streptomyces violaceoruber. European Journal of Biochemistry 39:201–205
    [Google Scholar]
  12. Tang T. 1977; Fructose uptake activity in Streptomyces venezuelae. FEMS Microbiology Letters 1:183–186
    [Google Scholar]
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