RT Journal Article SR Electronic(1) A1 Sietsma, J. H. A1 Wessels, J. G. H.YR 1981 T1 Solubility of (1→3)-β-d/(1→6)-β-d-Glucan in Fungal Walls: Importance of Presumed Linkage between Glucan and Chitin JF Microbiology, VO 125 IS 1 SP 209 OP 212 DO https://doi.org/10.1099/00221287-125-1-209 PB Microbiology Society, SN 1465-2080, AB In Saccharomyces cerevisiae, Neurospora crassa, Aspergillus nidulans and Coprinus cinereus most of the alkali-insoluble (1→3)-β-D/(1→6)-β-d-glucan of the wall can be extracted with dimethyl sulphoxide. The same fraction, and in Saccharomyces cerevisiae a small additional fraction, can be extracted by a destructive procedure involving 40% NaOH at 100 C. The small fraction of the glucan which resists this treatment becomes soluble after a subsequent treatment with HNO2 indicating that it is covalently linked to chitin in the wall. In contrast, in Schizophyllum commune and Agaricus bisporus, nearly all the (1→3)-βD-/(1→6)β-d-glucan appears to be held insoluble by linkage to chitin., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-125-1-209