In and most of the alkali-insoluble (13)-β-D/(16)-β-D-glucan of the wall can be extracted with dimethyl sulphoxide. The same fraction, and in a small additional fraction, can be extracted by a destructive procedure involving 40% NaOH at 100 C. The small fraction of the glucan which resists this treatment becomes soluble after a subsequent treatment with HNO2 indicating that it is covalently linked to chitin in the wall. In contrast, in and , nearly all the (1 3)-β-D-/(1 6)-β-D-glucan appears to be held insoluble by linkage to chitin.


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