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A test for susceptibility to 5-fluorouracil was useful for differentiating between species of rapidly growing mycobacteria, and for differentiating the genus Rhodococcus from the genus Nocardia. The majority of rhodococci tested were susceptible to 5-fluorouracil (20 μg ml−1), whereas the majority of nocardiae tested were resistent to it. Strains of Nocardia asteroides sensu stricto could be divided into two subgroups by their reaction to 5-fluorouracil.
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