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Isolated rabbit brush borders were used to investigate the adhesive properties of clinical and environmental isolates of non-O1 Vibrio cholerae and clinical isolates of Vibrio parahaemolyticus and Aeromonas hydrophila. A minority of the isolates were found to adhere to brush borders. The adhesion of these isolates was affected by a number of factors, including the bacteria: brush border ratio, incubation time, culture medium and growth temperature. Adhesion was inhibited by L-fucose but was independent of calcium ion concentration.