1887

Abstract

Sucrose and glucose supported acetylene reduction by bacteroids extracted from Frenchbean, soybean and pea root nodules in the presence of low O2 concentrations in experiments carried out with or without a gas phase, but not under O tensions usually able to support acetylene reduction with succinate. Addition of leghaemoglobin to the bacteroid suspensions allowed maximum rates of ethylene formation at very low O concentrations (1 to 5 n) when sucrose or glucose was present in assays with no gas phase. Stimulation of bacteroid O consumption was lower with these carbohydrates than with succinate for a similar acetylene reduction activity. This low O uptake provided O steady-state conditions in gas phase experiments. The optimal O tension required for bacteroid acetylene reduction was lower when nodule age increased.

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1981-07-01
2024-12-12
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