pLE2451, a 24·5 megadalton conjugative plasmid from Neisseria gonorrhoeae, was capable of efficiently mobilizing gonococcal β-lactamase plasmids between gonococci and from gonococci to Haemophilus influenzae and restriction-deficient Escherichia coli. Donor strains of N. gonorrhoeae carrying pLE2451 were also found to be capable of mobilizing a variety of non-conjugative plasmids originally derived from enteric bacteria or Haemophilus species when such plasmids were resident in E. coli. Nevertheless, pLE2451 was not detected physically in E. coli or H. influenzae transconjugants. This suggests that the plasmid is unstable in these hosts but survives transiently to provide transfer functions for mobilization. The proficiency of pLE2451 in promoting intraspecific and intergeneric mobilization was not paralleled by pUB701, pR1234 and pFR16017, a series of conjugative plasmids derived originally from Haemophilus species. These plasmids were incapable of mobilizing even Haemophilus -lactamase plasmids, such as RSF0885, between Haemophilus species.
AndersonE. S., ThrelfallE. J., CarrJ., McconnellM., SmithH. R.1977; Clonal distribution of resistance plasmid-carrying Salmonella typhimurium, mainly in the Middle East. Journal of Hygiene 72:471–487
BrownM. G. M., WestonA., SaundersJ. R., HumphreysG. O.1979; Transformation of Escherichia coli C600 by plasmid DNA at different phases of growth. FEMS Microbiology Letters 5:219–222
DaviesJ. K., NormarkS.1980; A relationship between plasmid structure, structural lability, and sensitivity to site-specific endonucleases in Neisseria gonorrhoeae. Molecular and General Genetics 177:251–260
ElwellL. P., RobertsM., MayerL. W., FalkowS.1977a; Plasmid-mediated beta-lactamase production in Neisseria gonorrhoeae. Antimicrobial Agents and Chemotherapy 11:528–533
ElwellL. P., SaundersJ. R., RichmondM. H., FalkowS.1911b; Relationships between some R-plasmids found in Haemophilus influenzae. Journal of Bacteriology 131:356–362
GrinstedJ., SaundersJ. R., IngramL. C., SykesR. B., RichmondM. H.1972; Properties of an R factor which originated in Pseudomonas aeruginosa 1822. Journal of Bacteriology 110:529–537
KaulfersP.-M., LaufsR., JahnG.1978; Molecular properties of transmissible R factors of Haemophilus influenzae determining tetracycline resistance. Journal of General Microbiology 105:243–252
LaufsR., KaulfersP.-M., JahnG., TeschnerU.1979; Molecular characterization of a small Haemophilus influenzae plasmid specifying β-lactamase and its relationship to R factors from Neisseria gonorrhoeae. Journal of General Microbiology 111:223–231
MeyersJ. A., SanchezD., ElwellL. P., FalkowS.1976; Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. Journal of Bacteriology 127:1529–1537
RobertsM., ElwellL. P., FalkowS.1977; Molecular characterization of two beta-lactamase-specifying plasmids isolated from Neisseria gonorrhoeae. Journal of Bacteriology 131:557–563
SaundersJ. R., ElwellL. P., FalkowS., SykesR. B., RichmondM. H.1978; β-Lactamases and R plasmids of Haemophilus influenzae. Scan-dinavian Journal of Infectious Diseases, Suppl. 13:16–22
SmithH. R., HumphreysG. O., AndersonE. S.1974; Genetic and molecular characterization of some non-transferring plasmids. Molecular and General Genetics 129:229–242
SparlingP. F., SoxT. E., MohammedW., GuymanL. F.1978; Antibiotic resistance in the gonococcus: diverse mechanisms of coping with a hostile environment. In Immunobiology of Neisseria gonorrhoeae pp. 44–52BrooksG. F., GotschlichE. C., HolmesK. K., SawyerW. D., YoungF. E. Edited by Washington: American Society for Micro-biology.;
Van KlingerenB., Van EmbdenJ., Dessens-KroonM.1977; Plasmid-mediated chloramphenicol resistance in Haemophilus influenzae. Antimicrobial Agents and Chemotherapy 11:383–387
WoodW. B.1966; Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA. Journal of Molecular Biology 16:118–133