Proteolytic Activities of a Rumen Bacterium, R8/4 Free

Abstract

Proteolysis of leaf Fraction 1 protein, casein, azocasein and bovine serum albumin by the cell-associated proteinases of the rumen bacterium R8/4 was investigated and the kinetic parameters and were evaluated for each substrate. A variety of proteinase inhibitors was used to show that the proteolytic activity comprises a mixture of at least three different classes of proteinase. With respect to substrate specificity and some inhibition characteristics, the proteolytic activity of R8/4 was similar to that of rumen contents.

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1981-07-01
2024-03-28
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