1887

Abstract

The morphology and protein composition of gas vesicles isolated from wild-type and gas vacuole defective (gv) isolates of strain 5 have been analysed. Two general vesicle shapes were found: spindles, and cylinders with short biconical ends. Wild-type strains contained a majority of spindle-shaped vesicles (94 %), while 69% of the vesicles in gv strains were cylindrical. Electrophoretic analysis of gas vesicle proteins showed that vesicle preparations contained only a single major protein species. In addition, the polypeptide in vesicles from the gv strain migrated more slowly than that from the wild-type strain. From the rates of electrophoretic migration, the molecular weights of gas vacuole proteins in the wild-type and gv strains were estimated as 16 800 and 18200, respectively.

Data provided here are consistent with the suggestion that polypeptide processing may be involved in the formation and assembly of gas vesicles in .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-125-1-103
1981-07-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/125/1/mic-125-1-103.html?itemId=/content/journal/micro/10.1099/00221287-125-1-103&mimeType=html&fmt=ahah

References

  1. Cohen-Bazire G., Kunisawa R., Pfennig N. 1969; Comparative study of the structure of gas vacuoles. Journal of Bacteriology 100:1049–1061
    [Google Scholar]
  2. Falkenberg P. 1974 Kjemisk karakterisering avgassvakuole membranen hos halobakterier. Thesis, Institutt for Teknisk Biokjemi Norges, Tekniske Høgskole, Universitetet i Trondheim, Norway.:
    [Google Scholar]
  3. Falkenberg P., Buckland B., Walsby A. E. 1972; Chemical composition of gas vesicles isolated from Anabaena flos-aquae. Archiv für Mikrobiologie 85:304–309
    [Google Scholar]
  4. Jones D. D., Jost M. 1971; Characterisation of the protein from gas vacuole membranes of the blue-green alga Microcystis aeruginosa. Planta 100:277–287
    [Google Scholar]
  5. Konopka A. E., Lara J. C., Staley J. T. 1977; Isolation and characterization of gas vesicles from Microcyclus aquaticus. Archives of Microbiology 112:133–140
    [Google Scholar]
  6. Lanyi J. K. 1974; Salt-dependent properties of proteins from extremely halophilic bacteria. Bacteriological Reviews 38:272–290
    [Google Scholar]
  7. Larsen H., Omang S., Steensland H. 1967; On the gas vacuoles of the halobacteria. Archiv für Mikrobiologie 59:197–203
    [Google Scholar]
  8. Simon R. D. 1978; Halobacterium strain 5 contains a plasmid which is correlated with the presence of gas vacuoles. Nature; London: 273314–317
    [Google Scholar]
  9. Simon R. D. 1980; Acrylamide gel electrophoresis of hydrophobic proteins: gas vacuole protein. Electrophoresis 1:172–176
    [Google Scholar]
  10. Stoeckenius W., Kunau W. H. 1968; Further characterisation of particulate fractions from lysed cell envelopes of Halobacterium halobium and isolation of gas vacuole membranes. Journal of Cell Biology 38:336–357
    [Google Scholar]
  11. Studier F. W. 1973; Analysis of bacteriophage T7 early RNAs and proteins on slab gels. Journal of Molecular Biology 79:237–248
    [Google Scholar]
  12. Waaland J. R., Branton D. 1969; Gas vacuole development in a blue-green alga. Science 163:1339–1341
    [Google Scholar]
  13. Walsby A. E. 1971; The pressure relationships of gas vacuoles. Proceedings of the Royal Society B178:301–326
    [Google Scholar]
  14. Walsby A. E. 1972; Structure and function of gas vacuoles. Bacteriological Reviews 36:1–32
    [Google Scholar]
  15. Walsby A. E. 1974; Isolation of gas vesicles from blue-green algae. Methods in Enzymology 31:678–686
    [Google Scholar]
  16. Walsby A. E. 1978; The gas vesicles of aquatic prokaryotes. Symposia of the Society for General Microbiology 28:327–358
    [Google Scholar]
  17. Williams R. C. 1953; The shapes and sizes of purified viruses as determined by electron microscopy. Cold Spring Harbor Symposia on Quantitative Biology 18:185–195
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-125-1-103
Loading
/content/journal/micro/10.1099/00221287-125-1-103
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error