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Summary: A mutant strain of Aspergillus parasiticus that accumulated the red mycelial pigment norsolorinic acid and low levels of aflatoxin was subjected to serial transfer in defined media. The isolation of unpigmented, non-aflatoxigenic forms was associated with conditions in which the inocula for serial transfers contained hyphae rather than conidia, but was not associated with either zinc deficiency or the presence of barium in the medium. The unpigmented, non-aflatoxigenic variants were stable.
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