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Cells of Bacillus subtilis NRRL B558 at early stages of sporulation contained considerable amounts of cysteic acid. The cell cysteic acid content began to increase at the onset of sporulation and was maximal when synthesis of dipicolinic acid commenced; it then decreased as sporulation advanced. Cysteic acid was synthesized from cysteine and metabolized to sulpholactate in the mother-cell cytoplasm, and the sulpholactate was then incorporated into the forespore. This metabolic change is considered to be a general phenomenon in spore-formers that contain sulpholactate in their spores.
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