1887

Abstract

SUMMARY: Crude extracts of grown on glucose, xylose or ethanol gave single peaks of classical transketolase activity following chromatography on columns of hydroxylapatite; the enzyme was heat-stable and showed no appreciable activity with formaldehyde as acceptor in place of ribose 5-phosphate. Extracts of methanol-grown cells showed two peaks of transketolase activity following chromatography on both hydroxylapatite and DEAE-cellulose. One peak was identified with that found for the cells grown on substrates other than methanol; the other peak showed dihydroxyacetone synthase activity in addition to transketolase activity. Both activities in the latter peak were very unstable and have been ascribed to one enzyme on the basis of identical rates of denaturation at all temperatures tested between 0 and 40 ¼C. It is suggested that this enzyme is a special transketolase synthesized only during methylotrophic growth of the yeast and, in contrast to classical transketolase, is capable of using equally well either formaldehyde or ribose 5-phosphate as glycolaldehyde acceptor. A method based on heat treatment has been suggested for the simultaneous assay of both transketolases present in crude extracts of a methylotrophically grown yeast.

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/content/journal/micro/10.1099/00221287-124-2-309
1981-06-01
2021-10-24
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References

  1. Dickens F., Williamson D.H. 1958; Formaldehyde as an acceptor aldehyde for transketolase, and the biosynthesis of triose. Nature; London: 1811790
    [Google Scholar]
  2. De La Haba G., Leder I.G., Racker E. 1955; Crystalline transketolase from baker’s yeast: isolation and properties. Journal of Biological Chemistry 214:409–426
    [Google Scholar]
  3. Kato N., Nishizawa T., Sakazawa C., Tani Y., Yamada H. 1979; Xylulose 5-phosphate dependent fixation of formaldehyde in a methanol-utilizing yeast Kloeckera sp. no. 2201. Agricultural and Biological Chemistry 43:2013–2015
    [Google Scholar]
  4. O’Connor M.L., Quayle J.R. 1980; Pentose phosphate-dependent fixation of formaldehyde by methanol-grown Hansenula polymorpha and Candida boidinii . Journal of General Microbiology 120:219–225
    [Google Scholar]
  5. Tabachnick M., Srere P.A., Cooper J., Racker E. 1958; Oxidative pentose phosphate cycle III. The interconversion of ribose 5-phosphate, ribulose 5-phosphate and xylulose 5-phosphate. Archives of Biochemistry and Biophysics 74:315–325
    [Google Scholar]
  6. Tani Y., Nishizawa T., Yamada H., Kato N., Sakazawa C. 1980; The first enzyme for the assimilation pathway of methanol in Kloeckera sp. no. 2201. 3rd International Symposium on Microbial Growth on C1-Compounds Sheffield, U.K.: (Abstract)
    [Google Scholar]
  7. Van Dijken J.P., Otto R., Harder W. 1976; Growth of Hansenula polymorpha in a methanol- limited chemostat.Physiological responses due to involvement of methanol oxidase as a key enzyme in methanol metabolism. Archives of Microbiology 111:137–144
    [Google Scholar]
  8. Van Dijken J.P., Harder W., Beardsmore A.J., Quayle J.R. 1978; Dihydroxy acetone: an intermediate in the assimilation of methanol by yeasts?. FEMS Microbiology Letters 4:97–102
    [Google Scholar]
  9. Waites M.J., Quayle J.R. 1980; Dihydroxy-acetone: a product of xylulose 5-phosphate-dependent fixation of formaldehyde by methanol-grown Candida boidinii . Journal of General Microbiology 118:321–327
    [Google Scholar]
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