1887

Abstract

Summary: A new serotype () of was isolated from the dental plaque of monkeys (). Serotype strains fermented mannitol and melibiose but not sorbitol or raffinose, failed to hydrolyse aesculin and arginine, did not produce hydrogen peroxide and were unable to grow in the presence of bacitracin at 2 units ml. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of whole-cell proteins showed serotype strains to be closely related to strains of genetic group III (i.e. serotypes and ). The serotype-specific antigen of serotype contained glucose and galactose but was antigenically distinct from the polysaccharide antigens of serotypes and . Serotype strains preferentially colonized developmental grooves of teeth and the proportion of serotype in the plaque flora was greater in monkeys fed a sucrose-rich diet than in monkeys fed a starch-based diet. A serotype strain was cariogenic for germ-free rats fed a high-sucrose diet, and serotype strains appear to be implicated in the caries process in monkeys.

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1981-06-01
2024-04-25
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References

  1. Beighton D., Hayday H. 1980; The effects of fluoride on the growth of oral streptococci. Microbios 27:117–124
    [Google Scholar]
  2. Beighton D., Miller W. A. 1977; A microbiological study of normal flora of macropod dental plaque. Journal of Dental Research 56:995–1000
    [Google Scholar]
  3. Bowen W. H. 1969; A vaccine against dental caries. A pilot experiment with monkeys (Macaca irus). British Dental Journal 126:159–160
    [Google Scholar]
  4. Bratthall D. 1969; Immunodiffusion studies on the serological specificity of streptococci resembling Streptococcus mutans . Odontologisk revy 20:231–243
    [Google Scholar]
  5. Bratthall D. 1970; Demonstration of five serological groups of streptococcal strains resembling Streptococcus mutans . Odontologisk revy 21:143–152
    [Google Scholar]
  6. Clarke J. K. 1924; On the bacterial factor in the aetiology of dental caries. British Journal of Experimental Pathology 5:141–147
    [Google Scholar]
  7. Cohen B., Bowen W. H. 1966; Dental caries in experimental monkeys. British Dental Journal 121:269–276
    [Google Scholar]
  8. Colman G. 1976; The viridans streptococci. In Selected Topics in Clinical Bacteriology pp. 179–198 de Louvois J. Edited by London: Baillière Tindall.;
    [Google Scholar]
  9. Colman G., Hayday H. 1980; A bacteriological study related to the onset of dental caries in monkeys (Macaca fascicularis) . Caries Research 14:285–297
    [Google Scholar]
  10. Colman G., Williams R. E. O. 1972; Taxonomy of some human viridans streptococci. In Streptococci and Streptococcal Diseases pp. 281–299 Wannamaker L., Matson J. M. Edited by London & New York: Academic Press.;
    [Google Scholar]
  11. Cornick D. E. R., Bowen W. H. 1972; The effect of sorbitol on the microbiology of dental plaque in monkeys (Macaca irus). Archives of Oral Biology 7:1637–1648
    [Google Scholar]
  12. Cowan S. T. 1974 Cowan and Steel’s Manual for the Identification of Medical Bacteria, 2nd edn.. Cambridge: Cambridge University Press.;
    [Google Scholar]
  13. Coykendall A. L. 1974; Four types of Streptococcus mutans based on their genetic, antigenic and biochemical characteristics. Journal of General Microbiology 83:327–338
    [Google Scholar]
  14. Facklam R. R. 1977; Physiological differentiation of viridans streptococci. Journal of Clinical Microbiology 5:184–201
    [Google Scholar]
  15. Gibbons R. J., van Houte J. 1975a; Dental caries. Annual Review of Medicine 26:121–136
    [Google Scholar]
  16. Gibbons R. J., van Houte J. 1975b; Bacterial adherence in oral microbial ecology. Annual Review of Microbiology 29:19–44
    [Google Scholar]
  17. Gold Q. G., Jordan H. V., van Houte J. 1973; A selective medium for Streptococcus mutans . Archives of Oral Biology 18:1357–1364
    [Google Scholar]
  18. Grenby T. H., Hutchinson J. B. 1969; The effects of diets containing sucrose, glucose or fructose on experimental dental caries in two strains of rats. Archives of Oral Biology 14:373–380
    [Google Scholar]
  19. Hamada S., Masuda N., Shimamoto T. 1979; Some biological properties of Streptococcus mutans isolated from human mouths, with reference to the correlation with serotypes. Archives of Oral Biology 24:627–631
    [Google Scholar]
  20. Hardie J. M., Bowden G. H. 1976; Physiological classification of oral viridans streptococci. Journal of Dental Research 55:A166–A176
    [Google Scholar]
  21. Hehre E. J., Neill J. M. 1946; Formation of serologically reactive dextran by streptococci from sub-acute bacterial endocarditis. Journal of Experimental Medicine 83:147–163
    [Google Scholar]
  22. Iacono V. J., Taubman M. A., Smith D. J., Levine M. J. 1975; Isolation and immunochemical characterization of the group-specific antigen of Streptococcus mutans 6715. Infection and Immunity 11:117–128
    [Google Scholar]
  23. Ikeda T., Sandham H. J. 1971; Prevalence of Streptococcus mutans on various tooth surfaces in Negro children. Archives of Oral Biology 16:1237–1240
    [Google Scholar]
  24. Keyes P. H. 1958; Dental caries in molar teeth of rats, II. A method for diagnosing and scoring several types of lesions simultaneously. Journal of Dental Research 37:1088–1099
    [Google Scholar]
  25. Keyes P. H. 1968; Similarities and differences in dental caries in various species. In Art and Science of Dental Caries Research pp. 185–199 Harris R. S. Edited by New York: Academic Press.;
    [Google Scholar]
  26. Kønig K. G., Marthaler T. M., Mühlemann H. R. 1958; Methodik der kurzfristigerzeugten Rattenkaries. Deutsche Zahn-, Mund- und Kiefer- heilkunde 29:99–127
    [Google Scholar]
  27. Krasse B. C. 1966; Human streptococci and experimental caries in hamsters. Archives of Oral Biology 11:429–436
    [Google Scholar]
  28. Linzer R., Slade H. D. 1974; Purification and characterization of Streptococcus mutans group d cell wall polysaccharide antigen. Infection and Immunity 10:361–368
    [Google Scholar]
  29. Little W. A., Korts D. C., Thomson L. A., Bowen W. H. 1977; Comparative recovery of Streptococcus mutans on ten isolation media. Journal of Clinical Microbiology 5:578–583
    [Google Scholar]
  30. Loesche W. J., Straffon L. H. 1979; Longitudinal investigation of the role of Streptococcus mutans in human fissure decay. Infection and Immunity 26:498–507
    [Google Scholar]
  31. McCabe R. M., Keyes P. H., Howell A. 1967; An in vitro method for assessing the plaque forming ability of oral bacteria. Archives of Oral Biology 12:1653–1656
    [Google Scholar]
  32. Menzies I. S., Mount J. N. 1975; Advantages of silica gel as a medium for rapid thin-layer chromatography of neutral sugars. Medical Laboratory Technology 32:269–276
    [Google Scholar]
  33. Niven C. F. Jr Smiley K. L., Sherman J. M. 1942; The hydrolysis of arginine by streptococci. Journal of Bacteriology 43:651–660
    [Google Scholar]
  34. Perch B., Kjems E., Ravn T. 1974; Biochemical and serological properties of Streptococcus mutans from various human and animal sources. Acta pathologica et microbiologica scandinavica B82:357–370
    [Google Scholar]
  35. Russell R. R. B. 1976; Classification of Streptococcus mutans strains by SDS gel electrophoresis. Microbios Letters 2:55–59
    [Google Scholar]
  36. Russell R. R. B. 1979a; Glucosyltransferases of Streptococcus niutans strain Ingbritt. Microbios 23:135–146
    [Google Scholar]
  37. Russell R. R. B. 1979b; Wall-associated protein antigens of Streptococcus mutans . Journal of General Microbiology 114:109–115
    [Google Scholar]
  38. Russell R. R. B. 1979c; Purification of Streptococcus mutans glucosyltransferase by polyethylene glycol precipitation. FEMS Microbiology Letters 6:197–199
    [Google Scholar]
  39. Russell R. R. B. 1979d; Comparison of oral Streptococcus mutans AHT with strains of serotypes a and g by biochemical and electrophoretic methods. Archives of Oral Biology 24:617–619
    [Google Scholar]
  40. Russell R. R. B. 1979e; Use of Triton X-100 to overcome the inhibition of fructosyltransferase by SDS. Analytical Biochemistry 97:173–175
    [Google Scholar]
  41. Shklair I. L., Keene M. J. 1974; A biochemical scheme for the separation of the five varieties of Streptococcus mutans . Archives of Oral Biology 19:1079–1081
    [Google Scholar]
  42. Socransky S. S., Manganiello A. D. 1971; The oral microbiota of man from birth to senility. Journal of Periodontology 42:485–496
    [Google Scholar]
  43. Spinell D. M., Gibbons R. J. 1974; Influence of culture medium on the glucosyltransferase and dextran-binding capacity of Streptococcus mutans6715 cells. Infection and Immunity 10:1448–1451
    [Google Scholar]
  44. Westphal O., Jann K. 1965; Bacterial lipo- polysaccharide extraction with phenol-water and further applications of the procedure. Methods in Carbohydrate Chemistry 5:83–91
    [Google Scholar]
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