1887

Abstract

Summary: Isolation of spiroplasmas from healthy and lethal yellowing-diseased coconut palms was attempted by passaging inoculum from palm tissues in a lactalbumin hydrolysate-based medium for up to 12 serial blind subcultures. Spiroplasmas were isolated in two initial experiments but these results could not be repeated in extensive further tests. Isolates grew well in conventional mycoplasma and spiroplasma media and showed cultural and morphological characteristics typical of the genus . Serological tests and electrophoretic analysis of cell protein preparations showed that the isolates differed from and the Corn Stunt spiroplasma. The trivial name spiroplasma is proposed to distinguish this organism which is represented by a reference strain, ATCC33287.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-124-2-263
1981-06-01
2021-05-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/124/2/mic-124-2-263.html?itemId=/content/journal/micro/10.1099/00221287-124-2-263&mimeType=html&fmt=ahah

References

  1. Barile M. F., Hopps H. E., Grabowski M. 1978; Incidence and sources of mycoplasma contamination: a brief review. In Mycoplasma Infection of Cell Cultures pp. 35–45 Edited by Mcgarrity G. J., Murphy D. G., Nichols W. W. New York & London: Plenum Press;
    [Google Scholar]
  2. Butler M., Knight B. C. J. G. 1960; The survival of washed suspensions of mycoplasma. Journal of General Microbiology 22:470–477
    [Google Scholar]
  3. Chen T. A., Liao C. H. 1975; Corn stunt spiroplasma: isolation, cultivation and proof of pathogenicity. Science 188:1015–1017
    [Google Scholar]
  4. Chiu R. J., Black L. M. 1967; Monolayer cultures of insect cell lines and their inoculation with a plant virus. Nature, London 215:1076–1078
    [Google Scholar]
  5. Clyde W. A. 1964; Mycoplasma species identification based upon growth inhibition by specific antisera. Journal of Immunology 92:958–965
    [Google Scholar]
  6. Cole R. M., Tully J. G., Popkin T. J., Bové J. M. 1973; Morphology, ultrastructure and bacteriophage infection of the helical mycoplasma-like organism (Spiroplasma citri, gen.nov., sp.nov.) cultured from ’stubborn’ disease of citrus. Journal of Bacteriology 115:367–386
    [Google Scholar]
  7. Cotty P. J., Chen T. A. 1979; Spiroplasma growth factors from chloroform extracted horse serum. Phytopathology 69:1025
    [Google Scholar]
  8. Davis R. E. 1979; Spiroplasmas: newly recognised arthropod-borne pathogens. In Leafhopper Vectors and Plant Disease Agents pp. 451–484 Edited by Maramorosch K., Harris K. F. New York & London: Academic Press;
    [Google Scholar]
  9. Davis R. E., Lee I. M., Basciano L. K. 1979; Spiroplasmas: serological groupings of strains associated with plants and insects. Canadian Journal of Microbiology 25:861–866
    [Google Scholar]
  10. Dollet M., Saillard C., Garcia-Jurado O., Vignault J. C., Gargani D., Tully J. G., Bové J. M. 1980; An approach to the serological study of the mycoplasmas of lethal yellowing in the coconuts in West Africa. In Proceedings of the 4th Meeting of the International Council on Lethal Yellowing, 1979 p. 8. University of Florida Publication FL-80-1.
    [Google Scholar]
  11. Eden-Green S. J. 1978; Attempts to extract and culture mycoplasmas from coconut palms. In Proceedings of the 3rd Meeting of the International Council on Lethal Yellowing, 1977 p. 20 University of Florida Publication FL-78-2
    [Google Scholar]
  12. Eden-Green S. J., Tully J. G. 1979; Isolation ofAcholeplasma spp. from coconut palms affected by lethal yellowing disease in Jamaica. Current Microbiology 2:311–316
    [Google Scholar]
  13. Eden-Green S. J., Markham P. G., Townsend R. 1980; Acholeplasmas and lethal yellowing disease II. Transmission experiments. In Proceedings of the 4th Meeting of the International Council on Lethal Yellowing, 1979 pp. 10–11 University of Florida Publication FL-80-1
    [Google Scholar]
  14. Freundt E. A., Andrews B. E., Ernø H., Kunze M., Black F. T. 1973; The sensitivity ofMycoplasmatales to sodium-polyanethol-sulphonate and digitonin. Zentralblatt für Bakteriologie, Parasitenkunde, lnfektionskrankheiten und Hygiene (Abteilung I, Originate A) 225:104–112
    [Google Scholar]
  15. Grylls N. E., Hunt P. 1971; A review of the study of the aetiology of coconut lethal yellowing disease. Oléagineux 26:311–315
    [Google Scholar]
  16. Hayflick L. 1965; Tissue cultures and mycoplasmas. Texas Reports on Biology and Medicine 23:285–303
    [Google Scholar]
  17. International Committee on Systematic Bacteriology, Subcommittee on the Taxonomy of Mollicutes 1979; Proposal of minimal standards for descriptions of new species of the classMollicutes . International Journal of Systematic Bacteriology 29:172–180
    [Google Scholar]
  18. Junca P., Saillard C., Tully J., Garcia-Jurado O., Degorce-Dumas J. -R., Mouches C., Vignault J. -C., Vogel R., Mccoy R., Whitcomb R., Williamson D., Latrille J., Bové J. M. 1980; Caracterisation de spiroplasmes isolés d’insectes et de fleurs de France continentale, de Corse et du Maroc; proposition pour une classification de spiroplasmes. Comptes rendus hebdomadaires de séances de l’Academie des sciences D290:1209–1212
    [Google Scholar]
  19. Kondo F., Maramorosch K., Mcintosh A. H., Varney E. H. 1977; Aster yellows spiroplasma: isolation and cultivationin vitro . Proceedings of the American Phytopathological Society 4190–191
    [Google Scholar]
  20. Markham P. G., Townsend R. 1979; Experimental vectors of spiroplasmas. In Leafhopper Vectors and Plant Disease Agents pp. 413–445 Edited by Maramorosch K., Harris K. F. New York & London: Academic Press;
    [Google Scholar]
  21. Markham P. G., Townsend R., Bar-Joseph M., Daniels M. J., Plaskitt A., Meddins B. M. 1974; Spiroplasmas are the causal agents of citrus little leaf disease. Annals of Applied Biology 78:49–57
    [Google Scholar]
  22. Markham P. G., Townsend R., Plaskitt K., Saglio P. 1977; Transmission of corn stunt to dicotyledonous plants. Plant Disease Reporter 61:342–345
    [Google Scholar]
  23. Mccoy R. E. 1978; Attempts to culture mycoplasmalike agents at Fort Lauderdale. In Proceedings of the 3rd Meeting of the International Council on Lethal Yellowing, 1977 p. 20. University of Florida Publication FL-78-2
    [Google Scholar]
  24. Mccoy R. E. 1979; Mycoplasmas and yellows diseases. In The Mycoplasmas 3 pp. 229–264 Edited by Whitcomb R. F., Tully J. G. New York & London: Academic Press;
    [Google Scholar]
  25. Nyland G., Raju B. C. 1978; Isolation and culture of a spiroplasma from pear trees affected by pear decline. Phytopathology News 12:216
    [Google Scholar]
  26. Raju B. C., Nyland G. 1978; Effects of different media on the growth and morphology of three newly isolated plant spiroplasmas. Phytopathology News 12:216
    [Google Scholar]
  27. Raju B. C., Purcell A. H., Nyland G. 1980; Isolation of spiroplasmas from leafhoppers exposed to aster yellows and X disease. In Proceedings of the 4th Meeting of the International Council on Lethal Yellowing, 1979 p. 12 University of Florida Publication FL-80-1
    [Google Scholar]
  28. Saglio P., Laflèche D., Bonissol C., Bové J. M. 1971; Isolement, culture et observation au microscope électronique des structures de type mycoplasme associées à la maladie du Stubborn des agrumes et leur comparaison avec les structures observées dans le cas de la maladie du Greening des agrumes. Physiologie végétate 9:569–582
    [Google Scholar]
  29. Saglio P., L’Hospital M., Laflèche D., Dupont G., Bové J. M., Tully J. G., Freundt E. A. 1973; Spiroplasma citri gen. and sp.n.: a mycoplasma-like organism associated with ’Stubborn’ disease of citrus. International Journal of Systematic Bacteriology 23:191–204
    [Google Scholar]
  30. Townsend R., Markham P. G., Plaskitt K. A. 1977; Multiplication and morphology ofSpiroplasma citri in the leafhopperEuscelis plebejus . Annals of Applied Biology 87:307–313
    [Google Scholar]
  31. Townsend R., Eden-Green S. J., Markham P. G., Archer D., Clark M. F. 1980; Achole- plasmas and lethal yellowing disease III. Microbiological and serological studies. In Proceedings of the 4th Meeting of the International Council on Lethal Yellowing, 1979 p. 11 University of Florida Publication FL-80-1
    [Google Scholar]
  32. Tully J. G., Rose D. L., Garcia-Jurado O., Vlgnault J. C., Saillard C., Bové J. M., Mccoy R. E., Williamson D. L. 1980; Serological analysis of a new group of spiroplasmas. Current Microbiology 3:369–372
    [Google Scholar]
  33. Waters H., Hunt P. 1980; Thein vivo three-dimensional form of a plant mycoplasma-like organism by the analysis of serial ultrathin sections. Journal of General Microbiology 116:111–131
    [Google Scholar]
  34. Whitcomb R. F., Williamson D. L. 1975; Helical wall-free prokaryotes in insects: multiplication and pathogenicity. Annals of the New York Academy of Sciences 266:260–275
    [Google Scholar]
  35. Williamson D. L., Whitcomb R. F. 1975; Plant mycoplasmas: a cultivable spiroplasma causes corn stunt disease. Science 188:1018–1020
    [Google Scholar]
  36. Williamson D. L., Whitcomb R. F., Tully J. G. 1978; The spiroplasma deformation test, a new serological method. Current Microbiology 1:203–207
    [Google Scholar]
  37. Williamson D. L., Tully J. G., Whitcomb R. F. 1979; Serological relationships of spiroplasmas as shown by combined deformation and metabolic inhibition tests. International Journal of Systematic Bacteriology 29:345–351
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-124-2-263
Loading
/content/journal/micro/10.1099/00221287-124-2-263
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error