SUMMARY: Isolation of spiroplasmas from healthy and lethal yellowing-diseased coconut palms was attempted by passaging inoculum from palm tissues in a lactalbumin hydrolysate-based medium for up to 12 serial blind subcultures. Spiroplasmas were isolated in two initial experiments but these results could not be repeated in extensive further tests. Isolates grew well in conventional mycoplasma and spiroplasma media and showed cultural and morphological characteristics typical of the genus . Serological tests and electrophoretic analysis of cell protein preparations showed that the isolates differed from and the Corn Stunt spiroplasma. The trivial name spiroplasma is proposed to distinguish this organism which is represented by a reference strain, ATCC 33287.


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