1887

Abstract

Protein synthesis during germination and appressorium formation of spores was investigated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and fluorography. Synthesis of polypeptides with molecular masses of 72, 43 and 38 kilodaltons was detected only in the 20000 supernatant fraction of spore homogenates at early stages (5—25 min) of incubation. These polypeptides are probably associated with the protein synthesis essential for spore germination that occurs within 40 min from the start of incubation. A polypeptide with a molecular mass of 95 kilodaltons was specifically synthesized only when appressoria were formed. When synthesis of proteins, including the 95 kilodalton polypeptide, was completely inhibited by cycloheximide added after 1 h incubation, appressoria matured in structure but not in function; they seemed to have no ability to penetrate artificial membranes.

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/content/journal/micro/10.1099/00221287-124-1-61
1981-05-01
2021-05-13
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