SUMMARY: Protein synthesis during germination and appressorium formation of spores was investigated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and fluorography. Synthesis of polypeptides with molecular masses of 72, 43 and 38 kilodaltons was detected only in the 20000 g supernatant fraction of spore homogenates at early stages (5--25 min) of incubation. These polypeptides are probably associated with the protein synthesis essential for spore germination that occurs within 40 min from the start of incubation. A polypeptide with a molecular mass of 95 kilodaltons was specifically synthesized only when appressoria were formed. When synthesis of proteins, including the 95 kilodalton polypeptide, was completely inhibited by cycloheximide added after 1 h incubation, appressoria matured in structure but not in function; they seemed to have no ability to penetrate artificial membranes.


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