SUMMARY: The susceptibility of Klebsiella aerogenes to cycloserine varied according to the growth conditions. In batch culture, cells were less susceptible to the antibiotic when glycine was present in the medium, presumably due to competition between glycine and cycloserine for the uptake system by which glycine, d-alanine and cycloserine are transported into the cell. In the chemostat at average dilution rates, ammonia-limited cultures were more susceptible to the antibiotic than were glucose-limited cultures. Under phosphate-limiting conditions cultures were at least ten times less susceptible. Under ammonia and phosphate limitation the susceptibility increased with increasing growth rate. The sensitivity of glucose-limited cells was independent of the growth rate. A high-affinity uptake system for cycloserine (as measured by d-alanine transport) was present in ammonia- and glucose-limited cells, but not in phosphate-limited cells. Thus, the phenotypically defined alterations in the susceptibility of the bacterium to cycloserine could be correlated with variations in its uptake system for the antibiotic.
BrownM.R.W.,
MellingJ.1969; Role of divalent cations in the action of polymyxin B and EDTA on Pseudomonas aeruginosa. Journal of General Microbiology 59:263–274
CurtissR.IIICharamellaL.J.,
BergC.M.,
HarrisP.E.1965; Kinetic and genetic analyses of D-cycloserine inhibition and resistance. In Escherichia coli. Journal of Bacteriology 90:1238–1250
EvansC.G.T.,
HerbertD.,
TempestD.W.1970; The continuous cultivation of microorganisms. 2. Construction of a chemostat. Methods in Microbiology 2:277–327
FinchJ.E,
Brown.M.R.W.
1975; The influence of nutrient limitation in a chemostat on the sensitivity of Pseudomonas aeruginosa to polymyxin and to EDTA. Journal of Antimicrobial Chemotherapy 1:379–386
GilbertP.,
BrownM.R.W.1978; Influence of growth rate and nutrient limitation on the gross cellular composition of Pseudomonas aeruginosa and its resistance to 3- and 4-chlorophenol. Journal of Bacteriology 133:1066–1072
JohnsonB.,
WhiteR.J.,
WilliamsonG.M.1978; Factors influencing the susceptibility of Candida albicans to the polyenoic antibiotics nystatin and amphotericin B. Journal of General Microbiology 104:325–333
MellingG.J,
BrownM.R.W.
1975; The effect of the bacterial environment on resistance. In Resistance of Pseudomonas aeruginosa pp. 35–69Brown.M. R. W.
Edited by London:: John Wiley.;
PankhurstC.E,
CraigA.S.
1979; Morphology and nitrogenase activity of agar cultures and root nodules formed by D-cycloserineresistant mutants of Rhizobium sp. strain 32H1. Journal OfCeneral Microbiology 110:177–184
WargelR.J.,
ShadurC.A.,
NeuhausF.C.1970; . Mechanism of D-cycloserine action: transport systems for D-alanine, D-cycloserine, L-alanine, and glycine. Journal of Bacteriology 103:778–788
WargelR.J.,
ShadurC.A.,
NeuhausF.C.1971; Mechanism of D-cycloserine action: transport mutants for D-alanine, D-cycloserine, and glycine. Journal of Bacteriology 105:1028–1035