1887

Abstract

The efficiency of a variety of common mutagens in producing mutation in P3 was examined. Ethyl methanesulphonate, methyl methanesulphonate, decarbamoyl mitomycin C, nitrous acid and gamma radiation did not mutate P3. -Methyl-′-nitro--nitrosoguanidine (MNNG) and ultraviolet radiation were both mutagenic, the former being the more effective. Transposon mutagenesis with Tn5 yielded the same frequency and range of auxotrophs as did MNNG.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-124-1-191
1981-05-01
2021-09-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/124/1/mic-124-1-191.html?itemId=/content/journal/micro/10.1099/00221287-124-1-191&mimeType=html&fmt=ahah

References

  1. Beringer J.E. 1973 Genetic studies with Rhizobium leguminosarum. Ph.D. thesis: University of East Anglia.;
    [Google Scholar]
  2. Beringer J.E. 1974; R factor transfer in Rhizobium leguminosarum. Journal of General Microbiology 84:188–198
    [Google Scholar]
  3. Beringer J.E., Beynon J.L., Buchanan-Wollaston A.V., Johnston A.W.B. 1978; Transfer of the drug-resistance transposon Tn5 to Rhizobium. Nature; London: 276633–634
    [Google Scholar]
  4. Botstein D., Kleckner N. 1977; Translocation and illegitimate recombination by the tetracycline resistance element Tn 10. In DNA Insertion Elements, Plasmids and Episomes pp. 185–204 Edited by Bukhari A. I., Shapiro J. A., Adhya S. L. New York: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  5. Boucher C., Bergeron B., Barate De Bertalmio M., Dénarié J. 1977; Introduction of bacteriophage Mu into Pseudomonas solanacearum and Rhizobium meliloti using the R factor RP4. Journal of General Microbiology 98:253–263
    [Google Scholar]
  6. Buchanan-Wollaston A.V., Beringer J.E., Brewin N.J., Hirsch P.R., Johnston A.W.B. 1980; Isolation of symbiotically defective mutants in Rhizobium leguminosarum by insertion of the transposon Tn5 into a transmissible plasmid. Molecular and General Genetics 178:185–190
    [Google Scholar]
  7. Drake J.W., Baltz R.H. 1976; The biochemistry of mutagenesis. Annual Review of Biochemistry 45:11–37
    [Google Scholar]
  8. Jacob A.E., Cresswell J.M., Hedges R.W., Coetzee J.N., Beringer J.E. 1976; Properties of plasmids constructed by the in vitro insertion of DNA from Rhizobium leguminosarum or Proteus mirabilis into RP4. Molecular and General Genetics 147:315–323
    [Google Scholar]
  9. Kaushik B.D., Venkataraman G.S. 1972a; Induced variation in microorganisms. I. The mutagenic effect of ultraviolet light on Rhizobium trifolii. Folia microbiologica 17:381–388
    [Google Scholar]
  10. Kaushik B.D., Venkataraman G.S. 1972b; Induced variation in microorganisms. II. The mutagenic effect of ethyl methanesulphonate on Rhizobium trifolii. Folia microbiologica 17:389–392
    [Google Scholar]
  11. Lorkiewicz Z., Zurkowski W., Kowalczuk E., Gorska-Melke A. 1971; Mutagenesis and conjugation in Rhizobium trifolii. Acta microbiologica polonica, Ser.A 3:101–107
    [Google Scholar]
  12. Meade H.M., Signer E.R. 1977; Genetic mapping of Rhizobium meliloti. Proceedings of the National Academy of Sciences of the United States of America 74:2076–2078
    [Google Scholar]
  13. Meade H.M., Ruvkun G., Sundaresan V., Aull S., Long S.R., Ausubel F.M. 1979; Genetic and molecular studies of the Rhizobium-legume symbiosis. In Genome Organisation and Expression in Plants. Abstracts of a N.A.T.O. Advanced Studies Institute/F.E.B.S. Advanced Course, University of Edinburgh.
    [Google Scholar]
  14. Raina J.L., Modi J.V. 1969; Genetic transformation in Rhizobium. Journal of General Microbiology 57:125–130
    [Google Scholar]
  15. Schwinghamer E.A., Dalmas R.L. 1961; Studies on induced variation in the rhizobia. II. Radiation sensitivity and induction of antibiotic-resistance markers. Applied Microbiology 9:410–414
    [Google Scholar]
  16. Sweet D.M., Moseley B.E.B. 1976; The resistance of Micrococcus radiodurans to killing and mutation by agents which damage DNA. Mutation Research 34:175–186
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-124-1-191
Loading
/content/journal/micro/10.1099/00221287-124-1-191
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error