1887

Abstract

Summary: sp. 1430/3 fixed N in the dark, but at a lower rate than in the light. Following the transfer of exponentially growing cultures to the dark, the rate of N fixation increased for between 2 and 5 h and then decreased, becoming negligible after 12 h. No substantial increase in activity occurred for about 10 h following re-illumination. It is suggested that the decrease in nitrogenase activity which occurred about 5 h after transfer to the dark was not caused by exhaustion of carbon reserves but by a cessation of nitrogenase synthesis coupled with an irreversible inactivation of the enzyme, probably by O. Subsequent recovery of activity apparently depended upon resynthesis of nitrogenase.

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1981-05-01
2024-04-24
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References

  1. Bone D. 1972; The influence of canavanine, O2 and urea on the steady-state levels of nitrogenase inAnabaena flos-aquae. Archiv für Mikrobiologie 86:13–24
    [Google Scholar]
  2. Bottomley P.J., Stewart W.D.P. 1977; ATP and nitrogenase activity in nitrogen-fixing heterocystous blue-green algae. New Phytologist 79:625–638
    [Google Scholar]
  3. Carr N.G. 1973; Metabolic control and autotrophic physiology. In The Biology of Blue-Green Algae pp. 39–65 Edited by Carr N.G., Whitton B.A. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  4. Fay P. 1965; Heterotrophy and nitrogen fixation inChlorogloea fritschii. Journal of General Microbiology 39:11–20
    [Google Scholar]
  5. Fay P. 1976; Factors influencing dark nitrogen fixation in a blue-green alga. Applied and Environmental Microbiology 31:376–379
    [Google Scholar]
  6. Gallon J.R., Kurz W.G.W., Larue T.A. 1973; Isocitrate supported nitrogenase activity inGloeocapsa sp. LB795. Canadian Journal of Microbiology 19:461–465
    [Google Scholar]
  7. Gallon J.R., Ul-Haque M.I., Chaplin A.E. 1978; Fluoroacetate metabolism inGloeocapsa sp. LB795 and its relationship to acetylene reduction (nitrogen fixation). Journal of General Microbiology 106:329–336
    [Google Scholar]
  8. Hamadi A.F., Gallon J.R. 1979; Studies on the role of calcium in acetylene reduction (nitrogen fixation) byGloeocapsa sp. 1430/3. Biochemical Society Transactions 7:1266–1267
    [Google Scholar]
  9. Mullineaux P.M., Chaplin A.E., Gallon J.R. 1980; Effects of a light to dark transition on carbon reserves, nitrogen fixation and ATP concentrations in cultures ofGloeocapsa (Gloeothece) sp. 1430/3. Journal of General Microbiology 120:227–232
    [Google Scholar]
  10. Murry M.A., Benemann J.R. 1979; Nitrogenase regulation inAnabaena cylindrica. Plant and Cell Physiology 20:1391–1401
    [Google Scholar]
  11. Stewart W.D.P., Haystead A., Dharmawhadene M.W.N. 1975; Nitrogen assimilation and metabolism in blue-green algae. In Nitrogen Fixation by Free-living Micro-organisms pp. 129–158 Edited by Stewart W.D.P. Cambridge: Cambridge University Press;
    [Google Scholar]
  12. Tözüm S.R.D., Gallon J.R. 1979; The effects of methyl viologen onGloeocapsa sp.LB795 and their relationship to the inhibition of acetylene reduction (nitrogen fixation) by oxygen. Journal of General Microbiology 111:313–326
    [Google Scholar]
  13. Tözüm S.R.D., Ul-Haque M.I., Chaplin A.E., Gallon J.R. 1977; The effect of fluoroacetate on acetylene reduction byGloeocapsa. Biochemical Society Transactions 5:1482–1484
    [Google Scholar]
  14. Watanabe A., Yamamoto Y. 1967; Heterotrophic nitrogen fixation by the blue-green algaAnabaenopsis circularis. Nature; London: 241738
    [Google Scholar]
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