1887

Abstract

Summary: The α and type pili produced by variants of P9 were isolated and characterized. The two types of pili showed clear differences in morphology, buoyant density (α, 1·292 g ml; , 1·282 g ml) and isoelectric point (α, pI 5·2; , pI 4·3). Amino acid analysis of α and pili showed that their overall composition was similar with the exception that pili had a higher content of glutamate and alanine residues. A high degree of structural homology was seen in two-dimensional peptide maps of tryptic hydrolysates of α and pili. The molecular differences between α and pili were reflected in their antigenic activity and in their ability to attach to human cells. The binding of α pili to buccal epithelial cells was pH dependent with a maximum binding of 49% at pH 6·5, whereas the attachment of pili showed no pH optimum. The binding of pili to erythrocytes differed from binding to buccal epithelial cells in that attachment of both pili was identical with no pH optimum. Data from attachment of pili to human cells suggest that erythrocytes lack receptors for binding gonococcal pili.

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1981-05-01
2024-04-25
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References

  1. Bates D.L., Perham R.N., Coggins J.R. 1975; Methods for obtaining peptide maps of proteins on a subnanomole scale. Analytical Biochemistry 68:175–184
    [Google Scholar]
  2. Brinton C.C., Bryan J., Dillon J.A., Guerina N., Jacobson L.J., Labik A., Lee S., Levine A., Lim S., Mcmichael J., Polen S., Rogers K., To A.C.-C., To S.C.-M. 1978; Uses of pili in gonorrhoea control: role of bacterial pili in disease, purification and properties of gonococcal pili, and progress in the development of a gonococcal pilus vaccine for gonorrhoea. In Immunobiology of Neisseria gonorrhoeae pp. 155–178 Brooks G. F., Gotschlich E. C., Holmes K. K., Sawyer W. D., Young. F. E. Edited by Washington, D.C.:: American Society for Microbiology;
    [Google Scholar]
  3. Buchanan T.M. 1975; Antigenic heterogeneity of gonococcal pili. Journal of Experimental Medicine 141:1470–1475
    [Google Scholar]
  4. Buchanan T.M., Chen K.C.S., Jones R.B., Hildebrandt J.F., Pearce W.A., Hermodson M.A., Newland J.C., Luchtel D.L. 1978; Pili and principal outer membrane protein of Neisseria gonorrhoeae: immunochemical, structural and pathogenic aspects. In Immunobiology of Neisseria gonorrhoeae pp. 145–154 Brooks G. F., Gotschlich E. C., Holmes K. K., Sawyer W. D., Young. F. E. Edited by Washington, D.C.:: American Society for Microbiology;
    [Google Scholar]
  5. Dilworth J.A., Hendley J.O., Mandell G.L. 1975; Attachment and ingestion of gonococci by human neutrophils. Infection and Immunity 11:512–516
    [Google Scholar]
  6. Greenwood F.C., Hunter W.M., Glover J.S. 1963; The preparation of 131I-labelled human growth hormone of high specific radioactivity. Biochemical Journal 89:114–123
    [Google Scholar]
  7. Heckels J.E., Blackett B., Everson J.S., Ward M.E. 1976; The influence of surface charge on the attachment of Neisseria gonorrhoeae to human cells. Journal of General Microbiology 96:359–364
    [Google Scholar]
  8. James J.F., Swanson J. 1978; Color/opacity colonial variants of Neisseria gonorrhoeae and their relationship to the menstrual cycle. In Immunobiology of Neisseria gonorrhoeae pp. 338–343 Brooks G. F., Gotschlich E. C., Holmes K. K., Sawyer W. D., Young. F. E. Edited by Washington, D. C.:: American Society for Microbiology;
    [Google Scholar]
  9. James A.N., Knox J.M., Williams R.P. 1976; Attachment of gonococci to sperm. Influence of physical and chemical factors. British Journal of Venereal Diseases 52:129–135
    [Google Scholar]
  10. Jephcott A.E., Reyn A., Birch-Anderson A. 1971; Neisseria gonorrhoeae III. Demonstration of presumed appendages to cells from different colony types. Acta pathologica et microbiologica scandin-avica B79:437–439
    [Google Scholar]
  11. Kroeks M.V.A.M., Kremer J. 1977; The pH in the lower third of the genital tract. In The Uterine Cervix in Reproduction, pp. 109–117 Insler V., Bettendorf. G. Edited by Stuttgart:: Thieme.;
    [Google Scholar]
  12. Kutsukake K., Iino T. 1980; A trans acting factor mediates inversion of a specific DNA segment in flagellar phase variation of Salmonella. Nature; London: 284:479–481
    [Google Scholar]
  13. Lambden P.R., Heckels J.E., James L.T., Watt P.J. 1979; Variations in surface protein composition associated with virulence properties in opacity types of Neisseria gonorrhoeae. Journal of General Microbiology 114:305–312
    [Google Scholar]
  14. Lambden P.R., Robertson J.N., Watt P.J. 1980; Biological properties of two distinct pilus types produced by isogenic variants of Neisseria gonorrhoeae P9. Journal of Bacteriology 141:393–396
    [Google Scholar]
  15. Niederwieser A. 1972; Thin-layer chromatography of amino acids and derivatives. Methods in Enzymology 25B:60–99
    [Google Scholar]
  16. Ofek I., Beachey E.H., Bisno A.L. 1974; Resistance of Neisseria gonorrhoeae to phagocytosis: relationship to colonial morphology and surface pili. Journal of Infectious Diseases 129:310–316
    [Google Scholar]
  17. Penke B., Ferenczi R., Kovacs K. 1974; A new acid hydrolysis method for determining tryptophan in peptides and proteins. Analytical Biochemistry 60:45–50
    [Google Scholar]
  18. Robertson J.N., Vincent P., Ward M.E. 1977; The preparation and properties of gonococcal pili. Journal of General Microbiology 102:169–177
    [Google Scholar]
  19. Salit I.E., Blake M., Gotschlich E.C. 1980; Intrastrain heterogeneity of gonococcal pili is related to opacity colony variance. Journal of Experimental Medicine 151:716–725
    [Google Scholar]
  20. Swanson J. 1973; Studies on gonococcus infection. IV. Pili: their role in attachment of gonococci to tissue culture cells. Journal of Experimental Medicine 137:571–589
    [Google Scholar]
  21. Swanson J., Kraus S.J., Gotschlich E.C. 1971; Studies on gonococcus infection. I. Pili and zones of adhesion: their relation to gonococcal growth patterns. Journal of Experimental Medicine 134:886–906
    [Google Scholar]
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