Sringent Control in an Extreme Thermophilic Bacterium. Preliminary Characterization of a Guanosine 3′,5′-Polyphosphate [(p)ppGpp] Synthesizing Enzyme from Thermus thermophilus

Ingrid Lienert; Dietmar Richter

doi:10.1099/00221287-123-2-383

Volume 123, Issue 2

Short Communication

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Sringent Control in an Extreme Thermophilic Bacterium. Preliminary Characterization of a Guanosine 3′,5′-Polyphosphate [(p)ppGpp] Synthesizing Enzyme from Thermus thermophilus

Ingrid Lienert¹, Dietmar Richter¹
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Affiliations: ¹ Institut fr Physiologische Chemie, Abteilung Zellbiochemie, Universitt Hamburg, 2 Hamburg 20, Martinistrasse 52, West Germany
Published: 01 April 1981 https://doi.org/10.1099/00221287-123-2-383

Abstract

A guanosine 3′,5′-bis(diphosphate) (ppGpp) and guanosine 3′-diphosphate 5′-triphosphate (pppGpp) synthesizing enzyme is present in the ribosomal fraction of Thermus thermophilus. The enzyme is comparable to the Escherichia coli (p)ppGpp synthetase I (stringent factor) in that it requires ribosomes, mRNA and codon-specific, uncharged tRNA. The ribosomal complex can be replaced by methanol. Ribosomes from Escherichia coli do not function as effectors of the Thermus thermophilus enzyme.