1887

Abstract

lectins interact with strains OB and OB, which possess B and H (O) blood group determinants, respectively. The interaction could be demonstrated by specific agglutination of the bacteria, by haemagglutination inhibition tests and by lectin-mediated peroxidase binding to the bacteria. The agglutination of OB by the galactose-binding lectin was inhibited by -galactose and by the lipopolysaccharide extracted from OB. Similarly, the specific agglutination of OB by the mannose-binding lectin (which also binds -fucose, -galactose and -fructose) was inhibited by -mannose, -fucose, -galactose and -fructose, as well as by the lipopolysaccharide extracted from OB. The interaction between OB and the mannose-binding lectin was also demonstrated by lectin-mediated peroxidase binding to the bacterial surface. Peroxidase binding was also inhibited by the above-mentioned sugars and OB lipopolysaccharide.

Treatment of cells of the two strains with protein-denaturing agents did not reduce their agglutination by the lectins. On the other hand, oxidation of the cell surface sugars by sodium metaperiodate or boiling the cells in the presence of 1 % acetic acid for 1 h abolished their agglutination by the two lectins. It is, therefore, suggested that the lectins interact with the B and H (O) blood group determinant sugars (-galactose in OB and -fucose in OB) residing in the lipopoly-saccharides of these strains.

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1981-04-01
2021-07-31
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References

  1. Ames B.N. 1966; Assay of inorganic phosphate, total phosphate and phosphatases. Methods in Enzymology 8:115–118
    [Google Scholar]
  2. Archibald A.R., Coapes H.E. 1971; The interaction of Concanavalin A with teichoic acids and bacterial walls. Biochemical Journal 123:665–667
    [Google Scholar]
  3. Archibald A.R., Coapes H.E. 1972; Blocking of bacteriophage receptor sites by Concanavalin A. Journal of General Microbiology 73:581–585
    [Google Scholar]
  4. Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  5. Garber N., Frimerman H. 1973; Resistance of enteropathogenic strains of Escherichia coli to penicillins and cephalosporins. Israel Journal of Medical Sciences 9:888–894
    [Google Scholar]
  6. Gilboa-Garber N. 1972; Inhibition of broad spectrum haemagglutinin from Pseudomonas aeruginosa by D-galactose and its derivatives. FEBS Letters 20:242–244
    [Google Scholar]
  7. Gilboa-Garber N., Mizrahi L., Garber N. 1972; Purification of the galactose-binding haemagglutinin of Pseudomonas aeruginosa by affinity chromatography using Sepharose. FEBS Letters 28:93–95
    [Google Scholar]
  8. Gilboa-Garber N., Mizrahi L., Garber N. 1977a; Mannose-binding haemagglutinins in extracts of Pseudomonas aeruginosa . Canadian Journal of Biochemistry 55:975–981
    [Google Scholar]
  9. Gilboa-Garber N., Nir-Mizrahi I., Mizrahi L. 1977b; Specific agglutination of Escherichia coli O128B12 by the mannose-binding proteins of Pseudomonas aeruginosa . Microbios 18:99–109
    [Google Scholar]
  10. Goldstein I.J., Hayes C.E. 1978; The lectins: carbohydrate-binding proteins of plants and animals. Advances in Carbohydrate Chemistry and Biochemistry 35:127–340
    [Google Scholar]
  11. Goldstein I.J., Misaki A. 1970; Interaction of Concanavalin A with arabinogalactan from the cell wall of Mycobacterium bovis . Journal of Bacteriology 103:422–425
    [Google Scholar]
  12. Hammarstrom S., Lindberg A.A., Robertsson E.S. 1972; Precipitation of lipopolysaccharides from rough mutants of Salmonella typhimurium by an A-hemagglutinin from Helix pomatia . European Journal of Biochemistry 25:274–282
    [Google Scholar]
  13. Karkhanis Y.D., Zeltner J.V., Jackson J.J., Carlo D.J. 1978; A new and improved micro-assay to determine 2-keto-3-deoxyoctonate in lipo-polysaccharide of Gram-negative bacteria. Analytical Biochemistry 85:595–601
    [Google Scholar]
  14. Le Minor L., Tournier P., Chalon A.M. 1973; Agglutinabilite par la Concanavaline A de diverse bacilles à Gram-négatif. Etude chez les Salmonella de la correlation avec la structure antigenique O. Annales de Microbiologie 124A:467–476
    [Google Scholar]
  15. Lis H., Sharon N. 1977; Lectins: their chemistry and application to immunology. In The Antigens 4 pp. 429–529 Edited by Sela M. New York: Academic Press;
    [Google Scholar]
  16. Lotan R., Sharon N., Mirelman D. 1975; Interaction of wheat-germ agglutinin with bacterial cells and cell-wall polymers. European Journal of Biochemistry 55:257–262
    [Google Scholar]
  17. Martinez-Palomo A., Gonzalez-Robles A., De La Torre M. 1973; Selective agglutination of pathogenic strains of Entamoeba histolytica induced by Con A. Nature New Biology 245:186–187
    [Google Scholar]
  18. Neu H.C., Heppel L.A. 1965; The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. Journal of Biological Chemistry 240:3685–3692
    [Google Scholar]
  19. Nicolson G.L. 1974; The interactions of lectins with animal cell surfaces. International Review of Cytology 39:89–190
    [Google Scholar]
  20. Orskov F., Orskov I., Jann B., Jann K., Muller-Seitz E., Westphal O. 1967; Immunochemis-try of Escherichia coli O antigens. Acta pathologica et microbiologica scandinavica 71:339–358
    [Google Scholar]
  21. Sabet S.F., Schnaitman C.A. 1971; Localization and solubilization of colicin receptors. Journal of Bacteriology 108:422–430
    [Google Scholar]
  22. Springer G.F. 1971; Blood-group and Forssman antigenic determinants shared between microbes and mammalian cells. Progress in Allergy 15:9–77
    [Google Scholar]
  23. West E.S., Todd W.R., Mason H.S., Van Bruggen J.T. 1967 Textbook of Biochemistry, 4th edn. p. 215 London: Collier-Macmillan;
    [Google Scholar]
  24. Westphal O., Jann K. 1965; Bacterial lipopoly-saccharides. Methods in Carbohydrate Chemistry 5:83–91
    [Google Scholar]
  25. Wiseman G.M., Caird J.D. 1977; Composition of the lipopolysaccharide of Neisseria gonorrhoeae . Infection and Immunity 16:550–556
    [Google Scholar]
  26. Wolpert J.S., Albersheim P. 1976; Hostsymbiont interactions. I. The lectins of legumes interact with the O-antigen-containing lipopoly-saccharides of their symbiont rhizobia. Biochemical and Biophysical Research Communications 70:729–737
    [Google Scholar]
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