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A class of chlorate-resistant mutants of Rhizobium japonicum 110 has been isolated which is devoid of nitrate reductase activity but forms normal amounts of this enzyme when the medium is supplemented with 100 μm-sodium molybdate. Addition of molybdate to the mutants in the presence of chloramphenicol results in the formation of active nitrate reductase detected by nitrite secretion in the culture supernatant.
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