1887

Abstract

An investigation of sediments from the littoral (shallow water) and profundal (deep water) zones of Blelham Tarn, a shallow eutrophic lake, showed marked differences in the microbial decomposition processes. These differences were due largely to differences in the degree of oxygenation, supply of electron acceptors, and mean summer temperature at the two sites. The changes in the hypolimnion (the deep water zone formed on thermal stratification, which may be treated essentially as a closed system) could be used to calculate profundal rates of aerobic respiration, NO and SO reduction, and methanogenesis, relative to the accumulation of CO Laboratory measurements demonstrated that NH accumulation, SO reduction and methanogenesis were more intense in the profundal than in the littoral zone. Anaerobic processes that occurred in the littoral sediments did so at greater depths than in the profundal sediments. The release of CH and N bubbles also provided estimates of the importance of these processes at the two sites. At both sites aerobic respiration was the most important component (about 50%) of carbon mineralization; SO reduction was the least important, accounting for only a small percentage of carbon turnover. Pathways of NO reduction and methanogenesis accounted for approximately equal proportions (varying between 15 and 25%) of the carbon mineralized. When the results were adjusted to account for the relative areas of the profundal and littoral zones, the former was the more important site of methanogenesis and SO reduction, whereas aerobic respiration and NO reduction were greater in the littoral zone. The major end-product of NO reduction was NH in the profundal and N in the littoral zone. The higher and continued levels of nitrification, which recycled the NH in the littoral sediments, were thought to contribute to this.

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1981-04-01
2021-05-11
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References

  1. Abdollahi H., Nedwell D.B. 1979; Seasonal temperature as a factor influencing bacterial sulfate reduction in a saltmarsh sediment. Microbial Ecology 5:73–79
    [Google Scholar]
  2. Abram J.N., Nedwell D.B. 1978a; Inhibition of methanogenesis by sulphate reducing bacteria competing for transferred hydrogen. Archives of Microbiology 117:89–92
    [Google Scholar]
  3. Abram J.N., Nedwell D.B. 1978b; Hydrogen as a substrate for methanogenesis and sulphate reduction in anaerobic saltmarsh sediment. Archives of Microbiology 117:93–97
    [Google Scholar]
  4. Balderstone W.L., Sherr B., Payne W.J. 1976; Blockage by acetylene of nitrous oxide reduction in Pseudomonas perfectomarinus . Applied and Environmental Microbiology 31:504–508
    [Google Scholar]
  5. Barber L.E., Ensign J.C. 1979; Methane formation and release in a small Wisconsin lake. Geomicrobiology Journal 1:341–353
    [Google Scholar]
  6. Benschneider K., Robinson R.J. 1952; A new spectrophotometric method for the determination of nitrite in seawater. Journal of Marine Research 11:87–96
    [Google Scholar]
  7. Billen G. 1976; Evaluation of nitrifying activity in sediments by dark 14C-bicarbonate incorporation. Water Research 10:51–57
    [Google Scholar]
  8. Burns N.M., Ross C. 1972; Project Hypo. (Canada Centre for Inland Waters Paper No. 6.). United States Environmental Protection Agency Technical Report No. TS-05-71-208-24.
    [Google Scholar]
  9. Cappenberg T.E., Jongenjan E. 1978; Microenvironments for sulfate reduction and methane production in freshwater sediments. In Environmental Biogeochemistry and Geomicrobiology. I. The Aquatic Environment pp. 129–138 Edited by Krumbein W.E. Michigan: Ann Arbor Science Publishers;
    [Google Scholar]
  10. Chan Y.K., Campbell N.E.R. 1980; Denitrification in Lake 227 during summer stratification. Canadian Journal of Fisheries and Aquatic Sciences 37:506–512
    [Google Scholar]
  11. Chaney A.L., Marbech E.P. 1962; Modified reagents for the determination of urea and ammonia. Clinical Chemistry 8:130–132
    [Google Scholar]
  12. Chatarpaul L., Robinson J.B., Kaushik N.K. 1980; Effects of tubificid worms on denitrification and nitrification in stream sediment. Canadian Journal of Fisheries and Aquatic Sciences 37:656–663
    [Google Scholar]
  13. Cole J.A., Brown C.M. 1980; Nitrite reduction to ammonia by fermentative bacteria: a short circuit in the biological nitrogen cycle. FEMS Microbiology Letters 7:65–72
    [Google Scholar]
  14. Delafontaine M.J., Naveau H.P., Nyns E.J. 1979; Fluorimetric monitoring of methanogenesis in anaerobic digesters. Biotechnology Letters 1:71–74
    [Google Scholar]
  15. Dunn G.M., Herbert R.A., Brown C.M. 1979; Influence of oxygen tension on nitrate reduction by a Klebsiella sp. growing in chemostat culture. Journal of General Microbiology 112:379–383
    [Google Scholar]
  16. Edmondson W.T. 1966; Changes in oxygen deficit of Lake Washington. Verhandlungen der Internationalen Vereinigung für theoretische und angewandte Limnologie 16:153–158
    [Google Scholar]
  17. Elliott R.J., Porter A.G. 1971; A rapid cadmium reduction method for the determination of nitrate in bacon and curing brines. Analyst 96:522–527
    [Google Scholar]
  18. Fallon R.D., Harrits S., Hanson R.S., Brock T.D. 1980; The role of methane in internal carbon cycling in Lake Mendota during summer stratification. Limnology and Oceanography 25:357–360
    [Google Scholar]
  19. Fenchel T.M., Jørgensen B.B. 1977; Detritus carbon, nitrogen and sulphur. Society for General Microbiology Quarterly 6:7–8
    [Google Scholar]
  20. Fenchel T., Blackburn T.H. 1979 Bacteria and Mineral Cycling London: Academic Press;
    [Google Scholar]
  21. Fenchel T.M., Jørgensen B.B. 1977; Detritus food chains of aquatic ecosystems: the role of bacteria. Advances in Microbial Ecology 1:1–58
    [Google Scholar]
  22. Goring C.A.I. 1962; Control of nitrification by 2-chloro-6-(trichloromethyl)pyridine. Soil Science 93:211–218
    [Google Scholar]
  23. Graetz D.A., Keeney D.R., Aspiras R.B. 1973; Eh status of lake sediment-water systems in relation to nitrogen transformations. Limnology and Oceanography 18:908–917
    [Google Scholar]
  24. Hall G.H., Collins V.G., Jones J.G., Horsley R.W. 1978; The effect of sewage effluent on Grasmere (English Lake District) with particular reference to inorganic nitrogen transformations. Freshwater Biology 8:165–175
    [Google Scholar]
  25. Hargrave B.T. 1969; Epibenthic algal production and community respiration of Marion Lake. Journal of the Fisheries Research Board of Canada 26:2003–2026
    [Google Scholar]
  26. Hargrave B.T. 1972; Aerobic decomposition of sediment and detritus as a function of particle surface area and organic content. Limnology and Oceanography 17:583–596
    [Google Scholar]
  27. Hayward P. 1968 Hypolimnetic oxygen demand and evolution of gas from lake bottoms Master’s Report, University of North Carolina; U.S.A.:
    [Google Scholar]
  28. Howard D.L., Frea J.I., Pfiester R.M. 1971; The potential for methane-carbon cycling in Lake Erie. In Proceedings of the 14th Conference on Great Lakes Research pp. 463–473
    [Google Scholar]
  29. Howarth R.W., Teal J.M. 1979; Sulfate reduction in a New England salt marsh. Limnology and Oceanography 24:999–1013
    [Google Scholar]
  30. Jones J.G. 1976; The microbiology and decomposition of seston in open water and experimental enclosures in a productive lake. Journal of Ecology 64:241–278
    [Google Scholar]
  31. Jones J.G. 1979a; Microbial activity in lake sediments with particular reference to electrode potential gradients. Journal of General Microbiology 115:19–26
    [Google Scholar]
  32. Jones J.G. 1979b; Microbial nitrate reduction in freshwater sediments. Journal of General Microbiology 115:27–35
    [Google Scholar]
  33. Jones J.G. 1980; Some differences in the microbiology of profundal and littoral lake sediments. Journal of General Microbiology 117:285–292
    [Google Scholar]
  34. Jones J.G., Simon B.M. 1979; The measurement of electron transport system activity in freshwater benthic and planktonic samples. Journal of Applied Bacteriology 46:305–315
    [Google Scholar]
  35. Jones J.G., Simon B.M. 1980; Decomposition processes in the profundal region of Blelham Tarn and the Lund tubes. Journal of Ecology 68:493–512
    [Google Scholar]
  36. Jones J.G., Downes M.T., Talling I.B. 1980; The effect of sewage effluent on denitrification in Grasmere (English Lake District). Freshwater Biology 10:341–359
    [Google Scholar]
  37. Jørgensen B.B. 1978; A comparison of methods for the quantification of bacterial sulfate reduction in coastal marine sediments. I. Measurement with radiotracer techniques. Geomicrobiology Journal 1:11–27
    [Google Scholar]
  38. Kamp-Nielsen L., Anderson J.M. 1977; A review of the literature on sediment: water exchange of nitrogen compounds. Progress in Water Technology 8:393–418
    [Google Scholar]
  39. Knowles R. 1979; Denitrification, acetylene reduction and methane metabolism in lake sediment exposed to acetylene. Applied and Environmental Microbiology 38:480–493
    [Google Scholar]
  40. Mackereth F.J.H. 1964; An improved galvanic cell for determination of oxygen concentrations in fluids. Journal of Scientific Instruments 41:38–41
    [Google Scholar]
  41. Mackereth F.J.H., Heron J., Talling J.F. 1978; Some revised methods of water analysis for limnologists. Freshwater Biological Association Scientific Publication No. 36.
    [Google Scholar]
  42. Moore R.L., Basset B.B., Swift M.J. 1979; Developments in the Remazol Brilliant Blue dyeassay for studying the ecology of cellulose decomposition. Soil Biology and Biochemistry 11:311–312
    [Google Scholar]
  43. Nedwell D.B., Abram J.W. 1979; Relative influence of temperature and electron donor and electron acceptor concentrations on bacterial sulfate reduction in saltmarsh sediments. Microbial Ecology 5:67–72
    [Google Scholar]
  44. Ohle W. 1956; Bioactivity, production and energy utilization of lakes. Limnology and Oceanography 1:139–149
    [Google Scholar]
  45. Pennington W. 1974; Seston and sediment formation in five Lake District lakes. Journal of Ecology 65:215–251
    [Google Scholar]
  46. Postgate J.R. 1963; Versatile medium for the enumeration of sulfate-reducing bacteria. Applied Microbiology 11:265–267
    [Google Scholar]
  47. Rees T.D., Gyllenspetz A.B., Docherty A.C. 1971; The determination of trace amounts of sulphide in condensed steam with N,N-diethyl-p- phenylenediamine. Analyst 96:201–208
    [Google Scholar]
  48. Richards F.A. 1965; Anoxic basins and fjords. In Chemical Oceanography 1 pp. 611–695 Edited by Riley J.P., Skirrow G. London: Academic Press;
    [Google Scholar]
  49. Robertson C.K. 1979; Quantitative comparison of the significance of methane in the carbon cycles of two small lakes. Ergebnisse der Limnologie 12:123–135
    [Google Scholar]
  50. Rudd J.W.M., Hamilton R.D. 1978; Methane cycling in a eutrophic shield lake and its effects on whole lake metabolism. Limnology and Oceanography 23:337–348
    [Google Scholar]
  51. Rudd J.W.M., Hamilton R.D. 1979; Methane cycling in Lake 227 in perspective with some components and oxygen cycles. Ergebnisse der Limnologie 12:115–122
    [Google Scholar]
  52. Sorensen J. 1978a; Denitrification rates in marine sediment as measured by the acetylene inhibition technique. Applied and Environmental Microbiology 36:139–143
    [Google Scholar]
  53. Sorensen J. 1978b; Capacity for denitrification and reduction of nitrate to ammonia in coastal marine sediment. Applied and Environmental Microbiology 35:301–305
    [Google Scholar]
  54. Stanier R.Y., Palleroni N.J., Doudoroff M. 1966; The aerobic pseudomonads: a taxonomic study. Journal of General Microbiology 43:159–271
    [Google Scholar]
  55. Strayer R.F., Tiedje J.M. 1978; In situ methane production in a small, hypereutrophic, hard-water lake: loss of methane from sediments by diffusion and ebullition. Limnology and Oceanography 23:1201–1206
    [Google Scholar]
  56. Tabatabai M.A. 1974; Determination of sulfate in water samples. Sulfur Institute Journal 10:11–13
    [Google Scholar]
  57. Wetzel R.G., Rich P.R., Miller M.C., Allen H.L. 1972; Metabolism of dissolved and particulate detrital carbon in a temperate hard-water lake. Memorie dell’Istituto italiano di idrobiologia Dott. Marco deMarchi 29 Supplement 185–243
    [Google Scholar]
  58. Winfrey M.R., Zeikus J.G. 1977; Effects of sulphate on carbon and electron flow during microbial methanogenesis in freshwater sediments. Applied and Environmental Microbiology 33:275–281
    [Google Scholar]
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