1887

Abstract

Antigens from a number of spiroplasmas were solubilized in the neutral detergent Lubrol PX and compared by crossed immunoelectrophoresis. Samples were also run in tandem with the type species , continuity of immunoprecipitin rockets indicating antigenic identity and serological relatedness of spiroplasmas. It was concluded that corn stunt spiroplasmas, the honeybee (BC3) spiroplasma and the tick (277F) spiroplasma were related serologically to but some flower isolates and the tick (SMCA) spiroplasma showed no obvious relationship. The serological relatedness of and the honeybee (BC3) spiroplasma was investigated in detail. These two spiroplasmas each contain a major membrane protein which differ slightly in molecular weight. The two proteins were purified under identical conditions and used to raise monospecific antisera. Using the sera obtained in this way with a modified immunoelectrophoretic system having sodium dodecyl sulphate-polyacrylamide gel electrophoresis as the first dimension separation, it was shown that the major membrane proteins of and the honeybee (BC3) spiroplasma are antigenically dissimilar. We suggest that spiralin, the major membrane protein of , and the major membrane protein of the honeybee (BC3) spiroplasma are dominant cell surface antigens, so that while these two spiroplasmas are known to be very similar, serological tests involving comparison of cell surface antigens indicate less relationship.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-123-1-61
1981-03-01
2021-07-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/123/1/mic-123-1-61.html?itemId=/content/journal/micro/10.1099/00221287-123-1-61&mimeType=html&fmt=ahah

References

  1. Archer D.B., Best J. 1980; Serological relatedness of spiroplasmas estimated by enzyme-linked immunosorbent assay and crossed immunoelectrophoresis. Journal of General Microbiology 119:413–422
    [Google Scholar]
  2. Christiansen C., Askaa G., Freundt E.A., Whitcomb R.F. 1979; Nucleic acid hybridization experiments with Spiroplasma citri and the corn stunt and suckling mouse cataract spiroplasmas. Current Microbiology 2:323–326
    [Google Scholar]
  3. Chua N.-H., Blomberg F. 1979; Immunochemical studies of thylakoid membrane polypeptides from spinach and Chlamydomonas reinhardtii. A modified procedure for crossed immunoelectrophoresis of dodecyl sulfate protein complexes. Journal of Biological Chemistry 254:215–223
    [Google Scholar]
  4. Davis R.E., Lee I.-M., Basciano L.K. 1979; Spiroplasmas:serological grouping of strains associated with plants and insects. Canadian Journal of Microbiology 25:861–866
    [Google Scholar]
  5. Denhardt D.T. 1966; A membrane filter tech-nique for the detection of complementary DNA. Biochemical and Biophysical Research Communications 23:641–646
    [Google Scholar]
  6. Fairbanks G., Steck T.L., Wallach D.F.H. 1971; Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry 10:2606–2617
    [Google Scholar]
  7. Hjertén S. 1963; Zone electrophoresis in columns of agarose suspensions. Journal of Chromatography 12:510–526
    [Google Scholar]
  8. Junca P., Saillard C., Tully J., Garcia-Jurado O., Degorce-Dumas J.R., Mouches C., Vignault J.C., Vogel R., Mccoy R., Whitcomb R., Williamson D., Latrille J., Bové J.M. 1980; Caracterisation de spiro-plasmes isolés d’insectes et de fleurs de France continentale, de Corse er du Maroc; proposition pour une classification/de spiroplasmes. Compte rendu hebdomadaire des séances de l’Académie des sciences D290:1209–1212
    [Google Scholar]
  9. Laemmli U.K., Favre M. 1973; Maturation of the head of bacteriophage T4.I.DNA packaging events. Journal of Molecular Biology 80:575–599
    [Google Scholar]
  10. Mouches C., Vignault J.C., Tully J.G., Whitcomb R.F., Bové J.M. 1979; Characterization of spiroplasmas by one and two dimensional protein analysis on polyacrylamide gel slabs. Current Microbiology 2:69–74
    [Google Scholar]
  11. Townsend R., Archer D.B., Plaskitt K.A. 1980; Purification and preliminary characterization of spiroplasma fibrils. Journal of Bacteriology 142:694–700
    [Google Scholar]
  12. Tully J.G., Rose D.L., Jurado O.G., Vignault J.C., Saillard C., Bové J.M., Mccoy R.E., Williamson D.L. 1980; Serological analysis of a new group of spiroplasmas. Current Microbiology 3:369–372
    [Google Scholar]
  13. Williamson D.L., Whitcomb R.F., Tully J.G. 1978; .The spiroplasma deformation test, a new serological method. Current Microbiology 1:203–207
    [Google Scholar]
  14. Williamson D.L., Tully J.G., Whitcomb R.F. 1979; .Serological relationships of spiroplasmas as shown by combined deformation and metabolism inhibition tests. International Journal of Systematic Bacteriology 29:345–351
    [Google Scholar]
  15. Wróblewski H. 1978; Spiralin: its topomolecular anatomy and its possible function in the Spiroplasma citri cell membrane. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I, Originale A) 241:179–180
    [Google Scholar]
  16. Wróblewski H. 1979; Amphiphilic nature of spiralin, the major protein of the Spiroplasma citri membrane . Journal of Bacteriology 140:738–741
    [Google Scholar]
  17. Wróblewski H., Johansson K.-E., Hjertén S. 1977; Purification and characterization of spiralin, the main protein of the Spiroplasma citri membrane. Biochimica et biophysica acta 465:275–289
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-123-1-61
Loading
/content/journal/micro/10.1099/00221287-123-1-61
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error