1887

Abstract

Accumulation of aminoglycoside antibiotics by bacteria requires energy, and it appears that this must be derived from electron transport occurring within the cytoplasmic membrane. Dependence of aminoglycoside accumulation on cellular menaquinone content was examined using a menaquinone auxotroph of . This dependence manifested itself only when the menaquinone concentration was decreased to less than 10% of normal. The restricted aminoglycoside accumulation observed under these conditions was closely correlated with susceptibility to growth inhibition by the antibiotics. Evidence of saturation of the accumulation system was observed at low menaquinone concentrations, an effect not seen when menaquinone deficiency was relieved by supplying adequate shikimic acid (a menaquinone precursor) to the auxotroph. Lipophilic quinones may play two roles in aminoglycoside accumulation by bacteria: (i) as a binding site or part of a carrier complex; and (ii) as a crucial component of the electron transport system in maintaining the proton electrochemical gradient.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-123-1-143
1981-03-01
2021-05-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/123/1/mic-123-1-143.html?itemId=/content/journal/micro/10.1099/00221287-123-1-143&mimeType=html&fmt=ahah

References

  1. Anagnostopoulos C., Spizizen J. 1961; Requirements for transformation in Bacillus subtilis . Journal of Bacteriology 81:741–746
    [Google Scholar]
  2. Bisschop A., Dejong L., Lima Costa M. E., Konings W. N. 1975; Relation between reduced nicotinamide adenine dinucleotide oxidation and amino acid transport in membrane vesicles from Bacillus subtilis . Journal of Bacteriology 121:807–813
    [Google Scholar]
  3. Bryan L. E., Van Den Elzen H. M. 1976; Streptomycin accumulation in susceptible and resistant strains of Escherichia coli and Pseudomonas aeruginosa . Antimicrobial Agents and Chemotherapy 9:928–938
    [Google Scholar]
  4. Bryan L. E., Van Den Elzen H. M. 1977; Effects of membrane-energy mutations and cations on streptomycin and gentamicin accumulation by bacteria: a model for entry of streptomycin and gentamicin in susceptible and resistant bacteria. Antimicrobial Agents and Chemotherapy 12:163–177
    [Google Scholar]
  5. Bryan L. E., Haraphongse R., Van Den Elzen H. M. 1976; Gentamicin resistance in clinical isolates of Pseudomonas aeruginosa associated with diminished gentamicin accumulation and no detectable enzyme modification. Journal of Antibiotics 29:743–753
    [Google Scholar]
  6. Bryan L. E., Kowànd S. K., Van Den Elzen H. M. 1979; Mechanism of aminoglycoside resistance in anaerobic bacteria: Clostridium perfringens and Bacteroides fragilis . Antimicrobial Agents and Chemotherapy 15:7–13
    [Google Scholar]
  7. Bryan L. E., Nicas T., Holloway B. W., Crowther C. 1980; Aminoglycoside-resistant mutation of Pseudomonas aeruginosa defective in cytochrome c552 and nitrate reductase. Antimicrobial Agents and Chemotherapy 17:71–79
    [Google Scholar]
  8. Farrand S. K., Taber H. W. 1973a; Pleiotropic menaquinone-deficient mutant of Bacillus subtilis . Journal of Bacteriology 115:1021–1034
    [Google Scholar]
  9. Farrand S. K., Taber H. W. 1973b; Physiological effects of menaquinone deficiency in Bacillus subtilis . Journal of Bacteriology 115:1035–1044
    [Google Scholar]
  10. Höltje J. -V. 1978; Streptomycin uptake via an inducible polyamine transport system in Escherichia coli . European Journal of Biochemistry 86:345–351
    [Google Scholar]
  11. Höltje J. -V. 1979a; Induction of streptomycin uptake in resistant strains of Escherichia coli . Antimicrobial Agents and Chemotherapy 15:177–181
    [Google Scholar]
  12. Höltje J. -V. 1979b; Regulation of polyamine and streptomycin transport during stringent and relaxed control in Escherichia coli . Journal of Bacteriology 137:661–663
    [Google Scholar]
  13. Muir M. E., Wallace B. J. 1979; Isolation of mutants of Escherichia coli uncoupled in oxidative phosphorylation using hypersensitivity to streptomycin. Biochimica et biophysica acta 547:218–229
    [Google Scholar]
  14. Newton N. A., Cox G. B., Gibson F. 1972; Function of ubiquinone in Escherichia coli: a mutantstrain forming a low level of ubiquinone. Journal of Bacteriology 109:69–73
    [Google Scholar]
  15. Plate C. 1976; Mutant of Escherichia coli defective in response to colicin K and in active transport. Journal of Bacteriology 125:467–474
    [Google Scholar]
  16. Taber H., Halfenger G. M. 1976; Multiple- aminoglycoside-resistant mutants of Bacillus subtilis deficient in accumulation of kanamycin. Antimicrobial Agents and Chemotherapy 9:251–259
    [Google Scholar]
  17. Taber H. W., Farrand S. K., Halfenger G. M. 1972; Genetic regulation of membrane components in Bacillus subtilis . In Spores V pp. 140–147 Edited by Halvorson H. O., Hanson R. S., Campbell L. L. Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  18. Taber H., Pomerantz B. J., Halfenger G. M. 1978; Near UV-induced growth delay studied in a menaquinone-deficient mutant of Bacillus subtilis . Photochemistry and Photobiology 28:191–196
    [Google Scholar]
  19. Thorbjarnardóttir S. H., Magnúsdóttir R. A., Eggertsson G., Kagan S. A., Andrésson O. S. 1978; Mutations determining generalized resistance to aminoglycoside antibiotics in Escherichia coli . Molecular and General Genetics 161:89–98
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-123-1-143
Loading
/content/journal/micro/10.1099/00221287-123-1-143
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error