@article{mbs:/content/journal/micro/10.1099/00221287-122-2-281, author = "Zwolshen, john H. and Bhattacharjee, J.K.", title = "Genetic and Biochemical Properties of Thialysine-resistant Mutants of Saccharomyces cerevisiae", journal= "Microbiology", year = "1981", volume = "122", number = "2", pages = "281-287", doi = "https://doi.org/10.1099/00221287-122-2-281", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-122-2-281", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Three groups of lysine-excreting, thialysine-resistant mutants of Saccharomyces cerevisiae were derived from the wild-type strain (X2180) by mutagenic treatment and selected on the basis of a cross-feeding assay. Mutants MNNG2-9, MNNG2-27, MNNG2-39 and MNNG2-62 (group I) exhibited a 2:2 segregation for thialysine resistance following mating with a wild-type strain and a lower than wild-type lysyl-tRNA synthetase activity; the thialysine-resistant phenotype was dominant in specific hybrids. Mutant MNNG2-2 (group II) was similar to group I mutants except that the thialysine-resistant phenotype was recessive in the hybrid. Mutant MNNG3-142 (group III) exhibited an irregular ratio of segregation of thialysine resistance and a significantly lower lysyl-tRNA synthetase activity; the thialysine-resistant phenotype was recessive in the hybrid. The growth of both group I and group III mutants was temperature-sensitive. The thialysine-resistant mutants exhibited pleiotropic properties including the increased production and excretion of lysine, thermosensitive growth and an impairment of lysyl-tRNA synthetase activity.", }