The labelling patterns of dihydroxyacetone and hexose phosphate produced after a 2 s incubation of methanol-grown with [C]methanol have been determined. In dihydroxyacetone over 90% of the radioactivity was located in C-1 and C-3 and less than 10% in C-2. In glucose over 99% of the radioactivity was evenly distributed between C-1, C-3, C-4 and C-6 with less than 1% in C-2 and C-5. The results are consistent with the operation of a previously proposed pentose phosphate cycle of formaldehyde fixation in which dihydroxyacetone is formed by glycolyl transfer from xylulose 5-phosphate to formaldehyde.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error