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The labelling patterns of dihydroxyacetone and hexose phosphate produced after a 2 s incubation of methanol-grown Hansenula polymorpha with [14C]methanol have been determined. In dihydroxyacetone over 90% of the radioactivity was located in C-1 and C-3 and less than 10% in C-2. In glucose over 99% of the radioactivity was evenly distributed between C-1, C-3, C-4 and C-6 with less than 1% in C-2 and C-5. The results are consistent with the operation of a previously proposed pentose phosphate cycle of formaldehyde fixation in which dihydroxyacetone is formed by glycolyl transfer from xylulose 5-phosphate to formaldehyde.
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