1887

Abstract

The labelling patterns of dihydroxyacetone and hexose phosphate produced after a 2 s incubation of methanol-grown with [C]methanol have been determined. In dihydroxyacetone over 90% of the radioactivity was located in C-1 and C-3 and less than 10% in C-2. In glucose over 99% of the radioactivity was evenly distributed between C-1, C-3, C-4 and C-6 with less than 1% in C-2 and C-5. The results are consistent with the operation of a previously proposed pentose phosphate cycle of formaldehyde fixation in which dihydroxyacetone is formed by glycolyl transfer from xylulose 5-phosphate to formaldehyde.

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/content/journal/micro/10.1099/00221287-122-2-193
1981-02-01
2024-04-19
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References

  1. Allen R.J.L. 1940; The estimation of phosphorus. Biochemical Journal 34:858–865
    [Google Scholar]
  2. Babel W., Loffhagen N. 1979; Assimilation of methanol by yeasts, a new approach. Zeitschrift für aligemeine Mikrobiologie 19:299–302
    [Google Scholar]
  3. Bartlett G.R. 1959; Methods for the isolation of glycolytic intermediates by column chromatography with ion-exchange resins. Journal of Biological Chemistry 234:459–465
    [Google Scholar]
  4. Fujii T., Tonomura K. 1973; Hexose phosphate as an intermediate in the assimilation of methanol by Candida sp. Agricultural and Biological Chemistry 37:447–449
    [Google Scholar]
  5. Fujii T., Asada Y. 1974; Assimilative pathway of methanol in Candida sp. Incorporation of l4C-methanol.14C formaldehyde. l4C-formate and 14C-bicarbonate into cell constituents. Agricultural and Biological Chemistry 38:1121–1127
    [Google Scholar]
  6. Kato N., Nishizawa T., Sakazawa C., Tani Y., Yamada H. 1979; XyIulose-5-phosphate dependent fixation of formaldehyde in methanol-utilizing yeast. Kloeckera sp. no. 2201. Agricultural and Biological Chemistry 43:2013–2015
    [Google Scholar]
  7. Kemp M.B., Quayle J.R. 1967; Microbial growth on C1 compounds. Uptake of I l4C Iformaldehyde and [l4C]formate by methane-grown Pseudomonas methanica and determination of the hexose labelling pattern after brief incubation with [14C] methanol. Biochemical Journal 102:94–102
    [Google Scholar]
  8. Large P.J., Peel D., Quayle J.R. 1961; Microbial growth on C, compounds. 2. Synthesis of cell constituents by methanol-and formate-grown Pseudomonas AM1.and methanol-grown Hvphomicrobiumv ulgare . Biochemical Journal 81:470–480
    [Google Scholar]
  9. Lewis K.F., Weinhouse S. 1957; Synthesis and degradation of isotopically labelled glycolic, glyoxylic and oxalic acids. Methods in Enzymology 4:609–614
    [Google Scholar]
  10. Lindley N.D., Waites M.J., Quayle J.R. 1980; A modified pulse-labelling technique for the detection of early intermediates in microbial metabolism: detection of [14C]dihydroxyacetone during assimilation of [14C] methanol by Hansenula polymorpha . FEMS Microbiology Letters 8:13–16
    [Google Scholar]
  11. Lowry O.H., Lopez J.A. 1946; The determination of inorganic phosphate in the presence of labile phosphate esters. Journal of Biological Chemistry 162:421–428
    [Google Scholar]
  12. O’Connor M.L., Quayle J.R. 1979; Mutants of Hansenula polymorpha and Candida boidinii impaired in their ability to grow on methanol. Journal of General Microbiology 113:203–208
    [Google Scholar]
  13. O’Connor M.L., Quayle J.R. 1980; Pentose phosphate-dependent fixation of formaldehyde by methanol-grown Hansenula polymorpha and Candida boidinii . Journal of General Microbiology 120:219–225
    [Google Scholar]
  14. Sakami W. 1955 Handbook of Isotope Tracer Methods Cleveland, Ohio: Department of Biochemistry, Case Western Reserve University School of Medicine.;
    [Google Scholar]
  15. Van Dijken J.P., Otto R., Harder W. 1976; Growth of Hansenula polymorpha in a methanol-limited chemostat.Physiological responses due to the involvement of methanol oxidase as a key enzyme in methanol metabolism. Archives of Microbiology 111:137–144
    [Google Scholar]
  16. Van Dijken J.P., Harder W., Beardsmore A.J., Quayle J.R. 1978; Dihydroxyacetone: an intermediate in the assimilation of methanol by yeasts?. FEMS Microbiology Letters 4:97–102
    [Google Scholar]
  17. Waites M.J., Quayle J.R. 1980; Dihydroxyacetone: a product of xylulose 5-phosphate-dependent fixation of formaldehyde by methanol-grown Candida boidinii . Journal of General Microbiology 118:321–327
    [Google Scholar]
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