SUMMARY: Intact cells of chemostat-grown (Bath) assimilated CO such that approximately 2.5% (w/w) of cell carbon arose from CO during growth with methane as carbon substrate. Radiolabelling studies suggested that CO was fixed by both ribulose-1,5-bisphosphate (RuBP) carboxylase and known heterotrophic mechanisms. The pattern of CO fixation was similar to that in heterotrophically grown autotrophs. Enzyme analysis suggested the presence of a complete Calvin cycle but attempts to grow the organism autotrophically were unsuccessful. A specific phosphoglycollate phosphatase, required for the metabolism of phosphoglycollate arising as a result of RuBP oxygenase activity, was present. The product of this reaction, glycollate, was further metabolized by reactions of the serine cycle, used for C assimilation in type II methylotrophs. The possession of this pathway explains both the presence of hydroxypyruvate reductase and the results of [C]formate uptake reported by other workers. The relationship between RuBP carboxylase/oxygenase and net carbon assimilation is discussed.


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