1887

Abstract

is normally grown in McCoy monolayer coverslip cultures using partially defined media containing foetal calf serum at concentrations up to 10% (v/v). Omission of the serum decreased the number of inclusions produced by infecting the McCoy cells with a standard inoculum of a genital strain of . Substitution of the foetal calf serum with a macromolecular fraction from the serum or with a mixture of sodium oleate, bovine serum albumin fraction V and fetuin maintained the inclusion count, but substitution with serum filtrate, containing the amino acids and other low molecular weight components, decreased the inclusion count of . The role of calf serum and the need for a fully defined medium excluding serum for studying the growth of in tissue culture are discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-122-1-47
1981-01-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/122/1/mic-122-1-47.html?itemId=/content/journal/micro/10.1099/00221287-122-1-47&mimeType=html&fmt=ahah

References

  1. Alexander J.J. 1968; Separation of protein synthesis in meningo-pneumonitis agent from that of L-cells by differential susceptibility to cycloheximide. Journal of Bacteriology 95:327–342
    [Google Scholar]
  2. Bader J.P., Morgan H.R. 1958; Latent virus infection of cells in tissue culture VI. Role of amino-acids, glutamine and glucose in psittacosis virus propagation in L-cells. Journal of Experi-mental Medicine 108:617–620
    [Google Scholar]
  3. Becker Y. 1978; The chlamydia: molecular biology of prokaryotic obligate parasites of eukaryocytes. Microbiological Reviews 42:274–306
    [Google Scholar]
  4. Bittles A.H. 1974; The comparative analysis of three batches of foetal bovine serum used in tissue culture. Medical Laboratory Technology 31:253–255
    [Google Scholar]
  5. Bushell A.C., Hobson D. 1978; Effect of cortisol on the growth of Chlamydia trachomatis in McCoy cells. Infection and Immunity 21:946–953
    [Google Scholar]
  6. Davies JA., Rees E., Hobson D., Karayiannis P. 1978; Isolation of Chlamydia trachomatis from Bartholin’s ducts. British Journal of Venereal Diseases 54:409–413
    [Google Scholar]
  7. Fisher H.W., Puck T.T., Sato G. 1958; Molecular growth requirements of single mammalian cells: the action of fetuin in promoting cell attachment to glass. Proceedings of the National Academy of Sciences of the United States of America 444–10
    [Google Scholar]
  8. Gordon F.B., Quan A.L. 1965; Isolation of the trachoma agent in cell culture. Proceedings of the Society for Experimental Biology and Medicine 118354–359
    [Google Scholar]
  9. Gordon F.B., Dressler H.R., Quan A.L., Mcquilkin W.T., Thomas J.I. 1972; Effect of ionizing irradiation on susceptibility of McCoy cultures to Chlamydia trachomatis. Applied Microbiology 23:123–129
    [Google Scholar]
  10. Hatch T.P. 1975; Competition between C. psittaci and L-cells for isoleucine pools. A limiting factor in chlamydial multiplication. Infection and Immunity 12:211–220
    [Google Scholar]
  11. Hobson D. 1977a; Non-gonococcal urethritis and related oculo-genital infections. Proceedings of the Royal Society of Medicine 7049–52
    [Google Scholar]
  12. Hobson D. 1977b; Tissue culture procedures for the isolation of Chlamydia trachomatis from patients with non gonococcal genital infections. In NonGonococcal Urethritis and Related Infections pp. 286–294 Edited by Hobson D., Holmes K.K. Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  13. Johnson F.W.A., Hobson D. 1976; Factors affecting the sensitivity of replicating McCoy cells in the isolation and growth of chlamydia A (TRIC agents). Journal of Hygiene 76:441–451
    [Google Scholar]
  14. Johnson F.W.A., Hobson D., Rees E., Tait I.A. 1977; Quantitative aspects of the growth of Chlamydia trachomatis in diagnostic tissue culture procedures. In Non-Gonococcal Urethritis and Related Infections pp. 309–313 Edited by Hobson D., Holmes K.K. Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  15. Morrison S.J., Jenkin H.M. 1972; Growth of Chlamydia psittaci strain meningo-pneumonitis in mouse L-cells cultivated in a defined medium in spinner cultures. In vitro 8:94–100
    [Google Scholar]
  16. Ripa K.T., Mårdh P.-A. 1977; Cultivation of Chlamydia trachomatis in cycloheximide-treated McCoy cells. Journal of Clinical Microbiology 6:328–331
    [Google Scholar]
  17. Rota T.R., Nichols R.L. 1973; Chlamydia trachomatis in cell culture. Applied Microbiology 26:560–565
    [Google Scholar]
  18. Sompolinsky D., Richmond S.J. 1974; Growth of Chlamydia trachomatis in McCoy cells treated with Cytochalasin B. Applied Microbiology 28:912–914
    [Google Scholar]
  19. Taylor W.G. 1974; ‘Feeding the baby’ - serum and other supplements to chemically defined medium. Journal of the National Cancer Institute 53:1448–1457
    [Google Scholar]
  20. Waymouth C. 1959; Rapid proliferation of sublines of NCTC clone 929 (strain L) mouse cells in a simple chemically defined medium MB752/1. Journal of the National Cancer Institute 22:1003–1017
    [Google Scholar]
  21. Wentworth B.B., Alexander E.R. 1974; Isolation of Chlamydia trachomatis by use of 5-iodo-2-deoxyuridine-treated cells. Applied Microbiology 27:912–916
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-122-1-47
Loading
/content/journal/micro/10.1099/00221287-122-1-47
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error