SUMMARY: is normally grown in McCoy monolayer coverslip cultures using partially defined media containing foetal calf serum at concentrations up to 10% (v/v). Omission of the serum decreased the number of inclusions produced by infecting the McCoy cells with a standard inoculum of a genital strain of . Substitution of the foetal calf serum with a macromolecular fraction from the serum or with a mixture of sodium oleate, bovine serum albumin fraction V and fetuin maintained the inclusion count, but substitution with serum filtrate, containing the amino acids and other low molecular weight components, decreased the inclusion count of . The role of calf serum and the need for a fully defined medium excluding serum for studying the growth of in tissue culture are discussed.


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