1887

Abstract

Growth of an oligosporogenic strain of T was studied in continuous culture. The maximum specific growth rate obtained was 0·47 h. RNA content increased linearly with increasing growth rate. Protein content decreased at high growth rates, but the DNA content of the culture was not affected by the growth rate. Activities of enzymes involved in glutamate utilization and energy production increased with increasing growth rate. The maximum specific activity for all enzymes assayed was obtained at a dilution rate of about 0.30 h. Changing the concentration of zinc or manganese in the medium by as little as 10 M from the concentration optimum for growth caused a significant reduction in the steady-state bacterial population. Zinc appeared to affect the efficiency of conversion of substrate to biomass. Manganese was very toxic at concentrations slightly above the concentration optimum for a specific growth rate.

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/content/journal/micro/10.1099/00221287-122-1-129
1981-01-01
2021-05-18
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