1887

Abstract

The changes in bacterial density, total extracellular protein and haemolysin produced by bacteria from overnight cultures of (Wood 46) and a low α-toxin-producing variant suspended in fresh medium were followed at 37 °C. Although five extracellular proteins were produced at a reduced level by the variant (α-toxin formation was reduced more than tenfold), the differential rates of total extracellular protein formation by the two organisms were identical. The results are consistent with a common regulatory mechanism for extracellular protein formation in which a pleiotropic compensation may occur in order to saturate the extracellular protein-producing capability.

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/content/journal/micro/10.1099/00221287-122-1-11
1981-01-01
2024-04-19
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References

  1. Abbas-Ali B., Coleman G. 1977a; The characteristics of extracellular protein secretion by Staphylococcus aureus (Wood 46) and their relationship to the regulation of a-toxin formation. Journal of General Microbiology 99:277–282
    [Google Scholar]
  2. Abbas-Ali B., Coleman G. 1977b; Nutritional shifts and their effect on the secretion of extracellular proteins by Staphylococcus aureus (Wood 46). Biochemical Society Transactions 5:420–422
    [Google Scholar]
  3. Bernheimer A. W., Schwartz L. L. 1963; Isolation and composition of staphylococcal alpha toxin. Journal of General Microbiology 30:455–468
    [Google Scholar]
  4. Coleman G., Abbas-Ali B. 1977; Comparison of the patterns of increase in α-toxin and total extracellular protein by Staphylococcus aureus (Wood 46) grown in media supporting widely differing growth characteristics. Infection and Immunity 17:278–281
    [Google Scholar]
  5. Coleman G., Brown S., Stormonth D. A. 1975; A model for the regulation of bacterial extracellular enzyme and toxin biosynthesis. Journal of Theoretical Biology 52:143–148
    [Google Scholar]
  6. Coleman G., Jakeman C. M., Martin N. 1978; Patterns of total extracellular protein secretion by a number of clinically isolated strains of Staphylococcus aureus. Journal of General Microbiology 107:189–192
    [Google Scholar]
  7. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  8. Monod J., Pappenheimer A. M., Cohen-Bazire G. 1952; La cinétique de la biosynthése de la β-galactosidase chez E. coli considérée comme fonction de la croissance. Biochimica et biophysica acta 9:648–660
    [Google Scholar]
  9. Sedmak J. J., Grossberg S. E. 1977; A rapid, sensitive and versatile assay for protein using Coomassie brilliant blue G250. Analytical Biochemistry 79:544–552
    [Google Scholar]
  10. Stormonth D. A., Coleman G. 1972; A rapid and convenient method for the determination of cell densities of bacteria which form aggregates. Journal of General Microbiology 71:407–408
    [Google Scholar]
  11. Yoshikawa M., Matsuda F., Naka M., Mirofushi E., Tsunematsu Y. 1974; Pleiotropic alteration of activities of several toxins and enzymes in mutants of Staphylococcus aureus. Journal of Bacteriology 119:117–122
    [Google Scholar]
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