1887

Abstract

Pyrolysis mass spectrometry (p.m.s.) was applied to differentiate 91 coded mycobacteria as belonging to either the ‘tuberculosis complex’ ( and BCG) or to other species of . Strains were analysed in batches; in each batch three strains each of and BCG were included as references. Mass spectra were evaluated by computerized multivariate analysis. A 92% positive correlation with classical identification tests was found (2·2% false negative, 5·5% false positive). Approaches for further improvement of this score are indicated. High speed and complete automation of sample analysis, as well as computerized data-processing, make p.m.s. a potential tool for routine application. Sample transfer from peripheral laboratories to a p.m.s. facility is easy, as samples can be sterilized before shipping.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-122-1-109
1981-01-01
2021-05-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/122/1/mic-122-1-109.html?itemId=/content/journal/micro/10.1099/00221287-122-1-109&mimeType=html&fmt=ahah

References

  1. Coster J.F., Manten A. 1956; A simple technique for the differentiation of tubercle bacilli and saprophytic mycobacteria. American Review of Tuberculosis and Pulmonary Diseases 74:958–960
    [Google Scholar]
  2. David H.L. 1978; Phenotypes of BCG-vaccines seed lot strains: results of an international cooperative study. Tubercle 59:139–142
    [Google Scholar]
  3. Eshuis W., Kistemaker P.G., Meuzelaar H.L.C. 1977; Some numerical aspects of reproducibility and specificity. In Analytical Pyrolysis pp. 151–166 Jones C.E.R., Cramers C.G.A. Edited by Amsterdam:: Elsevier.;
    [Google Scholar]
  4. Gruft H. 1976; Three simple tests as an adjunct to the niacin test for the small mycobacteriology laboratory. Health Laboratory Science 13:179–183
    [Google Scholar]
  5. Haider K., Nagar B.R., Saiz C., Meuzelaar H.L.C., Martin J.P. 1977; Studies on soil humic compounds, fungal melanins and model polymers by pyrolysis mass spectrometry. In Soil Organic Matter Studies II: pp. 213–220 Vienna:: International Atomic Energy Agency.;
    [Google Scholar]
  6. Kruskal J.B. 1964; Multidimensional scaling by optimizing goodness of fit to a nonmetric hypothesis. Psychometrica 29:1–29
    [Google Scholar]
  7. Kubica G.P. 1973; Differential identification of mycobacteria. VII. Key features for identification of clinically significant mycobacteria. American Review of Respiratory Disease 107:9–21
    [Google Scholar]
  8. Kubica G.P., Silcox V.A., Hall E. 1973; Numerical taxonomy of selected slowly growing mycobacteria. Journal of General Microbiology 74:159–167
    [Google Scholar]
  9. Laszlo A., Eidus L. 1978; Test for differentiation of M. tuberculosis and M. bovis from other mycobacteria. Canadian Journal of Microbiology 24:754–756
    [Google Scholar]
  10. Levy R.L. 1966; Pyrolysis gas chromatography, review of the technique. Chromatographic Reviews 8:48–89
    [Google Scholar]
  11. Mankiewicz E.M., Liivak M. 1971; Phage types of Mycobacterium bovis substrains of B.C.G. Canadian Journal of Microbiology 23:818–823
    [Google Scholar]
  12. Marks J. 1976; A new practical classification of the mycobacteria. Journal of Medical Microbiology 9:253–261
    [Google Scholar]
  13. Meissner G., Schrøder K., Amadio G.E., Anz W., Chaparas S.D., Engel H.W.B., Jenkins P.A., Kappler W., Kleeberg H.H., Kubala E., Kubin M., Lauterbach D., Lind A., Magnusson M., Mikova Z.D., Pattyn S.R., Schaefer W.B., Stanford J.L., Tsukamura M., Wayne L.G., Willers I., Wolinsky E. 1974; A cooperative numerical analysis of nonscoto- and nonphoto-chromogenic slowly growing mycobacteria. Journal of General Microbiology 83:207–235
    [Google Scholar]
  14. Meuzelaar H.L.C., Kistemaker P.G., Posthumus M.A. 1974; Recent advances in pyrolysis mass spectrometry of complex biological materials. Biomedical Mass Spectrometry 1:312–319
    [Google Scholar]
  15. Meuzelaar H.L.C., Kistemaker P.G., Tom A. 1975; Rapid and automated identification of microorganisms by Curie-point pyrolysis techniques. I. Differentiation of bacterial strains by fully automated Curie-point pyrolysis gas-liquid chromatography. In New Approaches to the Identification of Microorganisms pp. 165–178 Heden C.-G., Illéni T. Edited by New York:: Wiley.;
    [Google Scholar]
  16. Meuzelaar H.L.C., Kistemaker P.G., Eshuis W., Boerboom A.J.H. 1976; Automated pyrolysis mass spectrometry; application to the differentiation of microorganisms. In Advances in Mass Spectrometry B7 pp. 1452–1456 Daly N.R. Edited by London:: Heyden;
    [Google Scholar]
  17. Meuzelaar H.L.C., Kistemaker P.G., Eshuis W., Engel H.W.B. 1977; Progress in automated and computerized characterization of microorganisms by pyrolysis mass spectrometry. In Rapid Methods and Automation in Microbiology pp. 225–230 Newson S.W.B., Johnston H.H. Edited by Oxford:: Learned Information;
    [Google Scholar]
  18. Pattyn S.R., Portaels F. 1972; Identification and clinical significance of mycobacteria. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I, Originate A) 219:114–140
    [Google Scholar]
  19. Posthumus M.A., Boerboom A.J.H., Meuzelaar H.L.C. 1974; Analysis of biopolymers by Curie-point pyrolysis in direct combination with low voltage electron impact ionization mass spectrometry. In Advances in Mass Spectrometry 6 pp. 397–402 West A.R. Edited by London:: Heyden;
    [Google Scholar]
  20. Saito H., Gordon R.E., Jublin I., Käppler W., Kwapinksi J.B.G., McDurmont C., Pattyn S.R., Runyon E.H., Stanford J.L., Tarnok I., Tasaka H., Tsukamura M., Weiszfeiler J. 1977; Cooperative numerical analysis of rapidly growing mycobacteria. International Journal of Systematic Bacteriology 27:75–85
    [Google Scholar]
  21. Schulten H.-R., Gørtz W. 1978; Curie-point pyrolysis and field ionization mass spectrometry of polysaccharides. Analytical Chemistry 50:428–433
    [Google Scholar]
  22. Schulten H.-R., Beckey H.D., Meuzelaar H.L.C., Boerboom A.J.H. 1973; High resolution field ionization mass spectrometry of bacterial pyrolysis products. Analytical Chemistry 45:191–195
    [Google Scholar]
  23. Simmonds P.G. 1970; Whole microorganisms studied by pyrolysis gas chromatography-mass spectrometry: significance for extraterrestrial life detection experiments. Applied Microbiology 20:567–572
    [Google Scholar]
  24. Sneath P.H.A., Sokal R.R. 1973 Numerical Taxonomy. San Fransisco:: Freeman & Co;
    [Google Scholar]
  25. Tsukamura M. 1970; Screening for atypical mycobacteria. Tubercle 51:280–284
    [Google Scholar]
  26. Tsukamura M., Mizuno S. 1977; Numerical analysis of relationships among rapidly growing scotochromogenic mycobacteria. Journal of General Microbiology 98:511–517
    [Google Scholar]
  27. Wayne L.G., Dietz T.M., Gernez-Rieux C., Jenkins P.A., Kappler W., Kubica G.P., Kwapinsky J.B.G., Meissner G., Pattyn S.P., Runyon E.H., Schröder K.H., Silcox V.A., Tacquet A., Tsukamura M., Wolinsky E. 1971; A cooperative numerical analysis of scotochromogenic slowly growing mycobacteria. Journal of General Microbiology 66:255–271
    [Google Scholar]
  28. Wayne L.G., Engbaek H.C., Engel H.W.B., Froman S., Gross W., Hawkins J., Käppler W., Karlson A.G., Kleeberg H.H., Krasnow I., Kubica G.P., McDurmont C., Nel E.E., Pattyn S.R., Schröder K.H., Showalter S., Tarnok I., Tsukamura M., Vergmann B., Wolinsky E. 1974; Highly reproducible techniques for use in systematic bacteriology in the genus Mycobacterium : tests for pigment, urease, resistance to sodium chloride, hydrolysis of Tween 80 and β -galactosidase. International Journal of Systematic Bacteriology 24:412–419
    [Google Scholar]
  29. Wayne L.G., Engel H.W.B., Grassi C., Gross W., Hawkins J., Jenkins P.A., Käppler W., Kleeberg H.H., Krasnov I., Nel E.E., Pattyn S.R., Richards P.A., Showalter S., Slosarek M., Szabo I., Tarnok I., Tsukamura M., Vergmann B., Wolinsky E. 1976; Highly reproducible techniques for use in systematic bacteriology in the genus Mycobacterium: tests for niacin and catalase and for resistance to ioniazid, thiophene 2-carboxylic acid hydrazide, hydroxyl- amine and p -nitrobenzoate. International Journal of Systematic Bacteriology 26:311–318
    [Google Scholar]
  30. Wayne L.G., Andrade L., Froman S., Käppler W., Kubala E., Meissner G., Tsukamura M. 1978; A cooperative numerical analysis of Mycobacterium gastri, Mycobacterium kansasii and Mycobacterium marinum. . Journal of General Microbiology 109:319–327
    [Google Scholar]
  31. Weyman A.C.M. 1977; The application of Curie-point pyrolysis mass spectrometry in fungal taxonomy. In Analytical Pyrolysis pp. 225–233 Jones C.E.R., Cramers C.A. Edited by Amsterdam:: Elsevier;
    [Google Scholar]
  32. Wieten G., Haverkamp J., Engel H.W.B., Berwald L.G. 1980; Application of pyrolysis mass spectrometry in mycobacterial classification and identification. Reviews of Infectious Diseases 2: (in the Press).
    [Google Scholar]
  33. Windig W., Kistemaker P.C., Haverkamp J., Meuzelaar H.L.C. 1979; The effects of sample preparation, pyrolysis and pyrolyzate transfer conditions on pyrolysis mass spectra. Journal of Analytical and Applied Pyrolysis 1:39–52
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-122-1-109
Loading
/content/journal/micro/10.1099/00221287-122-1-109
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error