1887

Abstract

A cytosolic extract from the cellular slime mould contained an enzyme system that hydrolysed phosphatidylinositol. The route of degradation was exclusively by deacylation to glycerophosphoinositol. The hydrolysis was inhibited by phosphatidylethanolamine, the major phospholipid of the organism, which was also completely deacylated by myxamoebal extracts.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-121-2-495
1980-12-01
2021-05-09
Loading full text...

Full text loading...

/deliver/fulltext/micro/121/2/mic-121-2-495.html?itemId=/content/journal/micro/10.1099/00221287-121-2-495&mimeType=html&fmt=ahah

References

  1. Angus W. W., Lester F. L. 1972; Turnover of inositol and phosphorus containing lipids inSaccharomyces cerevisiae; extracellular accumulation of glycerophosphorylinositol derived from phosphatidylinositol. Archives of Biochemistry and Biophysics 151:483–495
    [Google Scholar]
  2. Bjerve K. S., Daae L. N. W., Bremer J. 1974; The selective loss of lysophospholipids in some commonly used lipid-extraction procedures. Analytical Biochemistry 58:238–245
    [Google Scholar]
  3. Brockerhoff H., Jensen R. G. 1974 Lipolytic Enzymes New York: Academic Press;
    [Google Scholar]
  4. Dawson R. M. C. 1959; Studies on the enzymic hydrolysis of monophosphoinositide by phospholipase preparations fromP. notatum and ox pancreas. Biochimica et biophysica acta 33:68–77
    [Google Scholar]
  5. Dawson R. M. C., Freinkel N., Jungawala F. B., Clarke N. 1971; The enzymic formation of myoinositol l:2-cyclic phosphate from phosphatidylinositol. Biochemical Journal 122:605–607
    [Google Scholar]
  6. Ellingson J. S. 1974; Changes in the phospholipid composition in differentiating cellular slime mould,Dictyostelium discoideum . Biochimica et biophysica acta 337:60–67
    [Google Scholar]
  7. Ferber E., Munder P. G., Fische H., Gerisch G. 1970; High phospholipase activities in amoebae ofDictyostelium discoideum . European Journal of Biochemistry 14:253–257
    [Google Scholar]
  8. Irvine R. F., Dawson R. M. C. 1980; The mechanism and function of phosphatidylinositol turnover. Biochemical Society Transactions 8:376–377
    [Google Scholar]
  9. Irvine R. F., Hemington N., Dawson R. M. C. 1977; . Phosphatidylinositol-degrading enzymes in liver lysosomes. Biochemical Journal 164:277–280
    [Google Scholar]
  10. Irvine R. F., Hemington N., Dawson R. M. C. 1978; . The hydrolysis of phosphatidylinositol by lysosomal enzymes of rat liver and brain. Biochemical Journal 176:475–484
    [Google Scholar]
  11. Irvine R. F., Hemington N., Dawson R. M. C. 1979; . The calcium-dependent phosphatidylino- sitol-phosphodiesterase of rat brain: mechanisms of suppression and stimulation. European Journal of Biochemistry 99:525–530
    [Google Scholar]
  12. Irvine R. F., Letcher A. J., Dawson R. M. C. 1980; Phosphatidylinositol phosphodiesterase in higher plants. Biochemical Journal 192:279–283
    [Google Scholar]
  13. Keenan R. W., Hokin L. E. 1964; The enzymic acylation of lysophosphatidylinositol. Journal of Biological Chemistry 239:2123–2129
    [Google Scholar]
  14. Machon A., North M. J., Brophy P. J. 1980; Biosynthesis of phosphatidylinositol in the cellular slime mouldDictyostelium discoideum by a CTP-independent pathway. Biochemical Society Transactions 8:375–376
    [Google Scholar]
  15. Richards D. E., Irvine R. F., Dawson R. M. C. 1978; Hydrolysis of membrane phospholipids by phospholipases of rat liver lysosomes. Biochemical Journal 182:599–606
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-121-2-495
Loading
/content/journal/micro/10.1099/00221287-121-2-495
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error