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A cytosolic extract from the cellular slime mould Dictyostelium discoideum contained an enzyme system that hydrolysed phosphatidylinositol. The route of degradation was exclusively by deacylation to glycerophosphoinositol. The hydrolysis was inhibited by phosphatidylethanolamine, the major phospholipid of the organism, which was also completely deacylated by myxamoebal extracts.
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