RT Journal Article SR Electronic(1) A1 Salcher, Olga A1 Lingens, FranzYR 1980 T1 Metabolism of Tryptophan by Pseudomonas aureofaciens and Its Relationship to Pyrrolnitrin Biosynthesis JF Microbiology, VO 121 IS 2 SP 465 OP 471 DO https://doi.org/10.1099/00221287-121-2-465 PB Microbiology Society, SN 1465-2080, AB Studies on the metabolism of tryptophan in Pseudomonas aureofaciens ATCC 15926 revealed different metabolic routes for the l-and d-isomer besides the biosynthetic pathway for pyrrolnitrin synthesis. l-Tryptophan catabolism follows the aromatic route via anthranilic acid. Tryptophan 2,3-dioxygenase was induced by l-tryptophan. Kynureninase and anthranilate 1,2-dioxygenase were induced by l-tryptophan, l-kynurenine and anthranilic acid. Anthranilate 1,2-dioxygenase was absent from a mutant strain of P. aureofaciens ATCC 15926 which produced about 30-fold increased amounts of pyrrolnitrin. The K m values of tryptophan 2,3-dioxygenase and kynureninase did not differ substantially between the two strains. Kynurenine 3-monooxygenase, 3-hydroxyanthranilate 3,4-dioxygenase, tryptophanase and indolyl-3-alkane α-hydroxylase activities were not detected. d- and l-tryptophan were converted to indole-3-pyruvate by tryptophan amino-transferase and via indole-3-acetaldehyde to indole-3-acetic acid. This additional catabolic pathway as well as tryptophan racemase activity was constitutive and present in both strains., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-121-2-465