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Haploid strains of Kluyveromyces lactis were used to study the factors that influence mating ability. Analysis of sterol extracts of cells showed that ergosterol acts like a mating-control sterol, depending on the frequency of subculture and the content of intracellular Ca2+. Mating reaction was abolished in yeast cells placed in contact with the ionophore A23187 when Ca2+was absent from the medium. Glucose at concentrations above 1% in the medium repressed the mating process; this repression could be reversed by either dibutyryl cyclic AMP or caffeine. In starved cells glucose stimulated mating ability if provided in the presence of oxygen; in these experiments cyclic AMP and caffeine had no effect.
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