1887

Abstract

Haploid strains of were used to study the factors that influence mating ability. Analysis of sterol extracts of cells showed that ergosterol acts like a mating-control sterol, depending on the frequency of subculture and the content of intracellular Ca. Mating reaction was abolished in yeast cells placed in contact with the ionophore A23187 when Cawas absent from the medium. Glucose at concentrations above 1% in the medium repressed the mating process; this repression could be reversed by either dibutyryl cyclic AMP or caffeine. In starved cells glucose stimulated mating ability if provided in the presence of oxygen; in these experiments cyclic AMP and caffeine had no effect.

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1980-12-01
2024-04-23
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